Effect of Different Carbon and Nitrogen Sources on Sclerotium rolfsii sacc. Mycelial Growth and Sclerotial Development

Fakher Ayed, Hayfa Jabnoun-Khiareddine, Rania Aydi-Ben Abdallah, Mejda Daami-Remadi


Different carbon and nitrogen sources were evaluated for their effects on the mycelial growth, and the sclerotial development of three Tunisian Sclerotium rolfsii Sacc. isolates. Radial growth was optimum on basal medium supplemented with ammonium chloride (0.48 g N.L-1) as N source, but was restricted on L-Arginine and completely inhibited on ammonium acetate amended media. Sclerotial initiation occurred from the 3rd to the 12th day of incubation for all tested isolates. Potassium nitrate was the most suitable N source for sclerotial formation whereas sclerotial development was completely inhibited on ammonium acetate amended medium. Optimal sclerotial germination was recorded using L-Arginine followed by L-Asparagine and ammonium chloride as N sources. Nevertheless, the lowest sclerotial germination rate was noted on sodium nitrate and ammonium acetate amended media. As for C sources (16 g C.L-1), optimal radial growth occurred using D-mannitol for Sr1 and Sr2 isolates and maltose for Sr3 but no mycelial growth was recorded using sodium citrate. All C sources tested, except sodium citrate, were suitable for sclerotial formation, production, and germination. Mature sclerotia became brownish after 6 to 12 days of incubation and sclerotial production was highest using D-mannitol, maltose and D-glucose, depending on isolates used, as C sources. Optimal germination of sclerotia was noted using D-glucose, D-mannitol and maltose for Sr1 isolate, maltose for Sr2 and D-glucose and maltose for Sr3. It was concluded that N and C sources are both important factors for the growth of S. rolfsii and its survival.


Carbon; germination; mycelial growth; nitrogen; sclerotial production; Sclerotium rolfsii


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DOI: 10.33687/phytopath.009.01.3066


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