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Assessment and Characterization of Colletotrichum species causing Bitter Rot Disease of Apple in Quetta (Pakistan)
Abstract
Colletotrichum species present a significant threat and posing a serious threat to the economy of Pakistan. This fact is due to suitable environmental conditions for pathogen dispersal. The research was conducted for the accurate identification of Colletotrichum species and studying its pathogenic behaviour responsible for pre-harvest bitter rot disease of apple fruits in Quetta, Pakistan. For this purpose, a survey was conducted in 2016 & 2017 across ten locations in Quetta to evaluate the disease assessment and sample collection. The findings revealed disease incidence of 39.22% in 2016 and 32.56% in 2017. Symptoms observed sunken brown lesions, measuring 1-4 cm in diameter, on the fruit surface, containing small, pinhead-sized black fruiting structures. A total of 130 fungal isolates were obtained from infected samples and categorized primarily into three groups (A, B, C) based on distinct cultural and morphological characteristics. Pathogenicity test, conducted in triplicate through spore suspension application on healthy apple fruit, confirmed Colletotrichum as the causative pathogen. Furthermore, ten isolates from each group were subjected to molecular analysis to identify the Colletotrichum species using internal transcribed spacer (ITS), beta-tubulin (TUB), and glyceraldehyde-3 phosphate dehydrogenase (GAPDH) gene regions. From sequence analysis, the fungal isolates were identified as three Colletotrichum species mainly Group (A) C. acutatum (B) C. gloeosporioides and (C) C. siamense. Morphological features act as a primary way of identifying Colletotrichum spp. although it is not feasible due to some Colletotrichum genera being morphologically related, consequently molecular method of identification was sufficient for accurate identification and species confirmation. At our knowledge, tion this is the first comprehensive study of Colletotrichum spp. causing bitter rot disease of apple in Pakistan. The findings of our resesearch will pave the way for future disease management experiments, aiming to prevent this disease becoming a serious threat in the region.
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References
Acheampong, F., A. N. Miller and M. Babadoost. 2024. Identifying species of pathogens causing bitter rot of apples in Illinois and efficacy of fungicides for managing the disease. HortTechnology, 34: 215-26.
Andrello, A. J., A. C. Velho, V. F. Soares, R. S. de Godoy, M. B. de Freitas, R. M. Valdebenito-Sanhueza, F. R. Kulcheski and M. J. Stadnik. 2024. Comparative virulence of Colletotrichum chrysophilum and Colletotrichum nymphaeae on apple leaves. Physiological and Molecular Plant Pathology, 131: 102285.
Arx, J. V. 1957. The species of the genus Colletotrichum Cda. Phytopathologische Zeitschrift, 29: 413-68.
Bailey, J. A. and M. J. Jeger. 1992. Colletotrichum: Biology, Pathology and Control. CAB International: Wallingford, UK.
Barrech, D., S. Bano, S. Bashir, H. Zafar and S. Naz. 2023. Balochistan's Potential under CPEC: Opportunities and Challenges. Qualitative Research, 23: 235-57.
Beever, R. E., T. L. Olsen and S. L. Parkes. 1995. Vegetative compatibility groups in Colletotrichum gloeosporioides (Glomerella cingulata) from apple and other fruits. Australasian Plant Pathology, 24: 126-32.
Ben Struble, F. and G. Keitt. 1950. Variability and inheritance in Glomerella cingulata (Stonem.) S. and VS from apple. American Journal of Botany, 37: 563-76.
Cabrefiga, J., D. Pizà, P. Vilardell and J. Luque. 2022. First report of Colletotrichum chrysophilum causing apple bitter rot in Spain. Plant Disease, 106: 1752.
Cai, L., K. Hyde, P. Taylor, B. Weir, J. Waller, M. Abang, J. Zhang, Y. Yang, S. Phoulivong and Z. Liu. 2009. A polyphasic approach for studying Colletotrichum. Fungal Diversity, 39: 183-204.
Cannon, P. F., P. Bridge and E. Monte. 2000. Linking the past, present and future of Colletotrichum systematics. APS Press: Saint Paul, Minnesota, USA.
Carneiro, G. A. and S. Baric. 2021. Colletotrichum fioriniae and Colletotrichum godetiae causing postharvest bitter rot of apple in South Tyrol (Northern Italy). Plant Disease, 105: 3118-26.
Caro, M. D. P., D. M. Pott, R. H. Tomas-Grau, M. Perato, P. L. Albornoz, J. C. Díaz-Ricci, S. Osorio, S. M. Salazar and S. Moschen. 2023. Defence responses triggered during the plant-pathogen interaction between strawberry (Fragaria x ananassa) and Colletotrichum acutatum. Plant Stress, 10: 100219.
Chen, Y., D. Fu, W. Wang, M. L. Gleason, R. Zhang, X. Liang and G. Sun. 2022. Diversity of Colletotrichum species causing apple bitter rot and Glomerella leaf spot in China. Journal of Fungi, 8: 740.
Crouch, J. A., B. B. Clarke, J. F. White Jr and B. I. Hillman. 2009. Systematic analysis of the falcate-spored graminicolous Colletotrichum and a description of six new species from warm-season grasses. Mycologia, 101: 717-32.
Damm, U., J. Woudenberg, P. Cannon and P. Crous. 2009. Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity, 39: 45.
De Silva, D., R. Mann, J. Kaur, P. Ekanayake, T. Sawbridge, S. McKay, P. Taylor and J. Edwards. 2021. Revisiting the Colletotrichum species causing anthracnose of almond in Australia. Australasian Plant Pathology, 50: 267-79.
Du, M., C. L. Schardl, E. M. Nuckles and L. J. Vaillancourt. 2005. Using mating-type gene sequences for improved phylogenetic resolution of Collectotrichum species complexes. Mycologia, 97: 641-58.
FAO. 2023. World Food and Agriculture Statistical year Book. Food and Agriculture Organization. Rome, Italy.
Fernandez, L. N., S. Alaniz, M. G. Derita, A. Lutz, N. F. Gariglio, P. E. Mondino and M. A. Favaro. 2022. Characterization of Colletotrichum siamense the causal agent of bitter rot and leaf spot in low-chill apple cultivars in central Argentina. International Journal of Pest Management, 68: 295-310.
González, E. and T. B. Sutton. 2004. Population diversity within isolates of Colletotrichum spp. causing Glomerella leaf spot and bitter rot of apples in three orchards in North Carolina. Plant Disease, 88: 1335-40.
GOP. 2023. Crops area and production (District wise), 2022-23. Ministry of National Food Security and Research, Economic Wing. Islamabad, Pakistan.
Grammen, A., M. Wenneker, J. Van Campenhout, K. Pham, W. Van Hemelrijck, D. Bylemans, A. Geeraerd and W. Keulemans. 2019. Identification and pathogenicity assessment of Colletotrichum isolates causing bitter rot of apple fruit in Belgium. European journal of plant pathology, 153: 47-63.
Hu, S., S. Zhang, W. Xiao, Y. Liu, H. Yu and C. Zhang. 2023. Diversity and characterization of resistance to pyraclostrobin in Colletotrichum spp. from strawberry. Agronomy, 13: 2824.
Ivic, D., D. Voncina, Z. Sever, S. Simon and I. Pejic. 2013. Identification of Colletotrichum species causing bitter rot of apple and pear in Croatia. Journal of Phytopathology, 161: 284-86.
Jayawardena, R., K. Hyde, U. Damm, L. Cai, M. Liu, X. Li, W. Zhang, W. Zhao and J. Yan. 2016. Notes on currently accepted species of Colletotrichum. Mycosphere, 7: 1192-260.
Khodadadi, F., J. B. González, P. L. Martin, E. Giroux, G. J. Bilodeau, K. A. Peter, V. P. Doyle and S. G. Aćimović. 2020. Identification and characterization of Colletotrichum species causing apple bitter rot in New York and description of C. noveboracense sp. nov. Scientific reports, 10: 11043.
Lee, D.-H., D.-H. Kim, Y.-A. Jeon, J.-Y. Uhm and S.-B. Hong. 2007. Molecular and cultural characterization of Colletotrichum spp. causing bitter rot of apples in Korea. The Plant Pathology Journal, 23: 37-44.
Martin, P. L., T. Krawczyk, F. Khodadadi, S. G. Aćimović and K. A. Peter. 2021. Bitter rot of apple in the Mid-Atlantic United States: Causal species and evaluation of the impacts of regional weather patterns and cultivar susceptibility. Phytopathology, 111: 966-81.
Martin, P. L. and K. A. Peter. 2023. Spore dispersal patterns of Colletotrichum fioriniae in orchards and the timing of apple bitter rot infection periods. Plant Disease, 107: 2474-82.
Munir, M., B. Amsden, E. Dixon, L. Vaillancourt and N. W. Gauthier. 2016. Characterization of Colletotrichum species causing bitter rot of apple in Kentucky orchards. Plant Disease, 100: 2194-203.
Nekoduka, S., K. Tanaka and T. Sano. 2018. Epidemiology of apple bitter rot caused by Colletotrichum acutatum sensu lato. Journal of General Plant Pathology, 84: 262-71.
Oo, M. M., H.-Y. Yoon, H. A. Jang and S.-K. Oh. 2018. Identification and characterization of Colletotrichum species associated with bitter rot disease of apple in South Korea. The Plant Pathology Journal, 34: 480.
Pakistan Meteorological Department. 2023. Environment data. Regional Meteorological Center. Quetta, Pakistan.
Peres, N., L. Timmer, J. Adaskaveg and J. Correll. 2005. Lifestyles of Colletotrichum acutatum. Plant Disease, 89: 784-96.
Powell, L. 1985. The chilling requirement in apple and its role in regulating time of flowering in spring in cold-winter climates. V International Symposium on Growth Regulators in Fruit Production 179.
Prihastuti, H., L. Cai, H. Chen, E. McKenzie and K. Hyde. 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity, 39: 89-109.
Riolo, M., A. Pane, E. Santilli, S. Moricca and S. O. Cacciola. 2023. Susceptibility of Italian olive cultivars to various Colletotrichum species associated with fruit anthracnose. Plant Pathology, 72: 255-67.
Salotti, I., T. Ji and V. Rossi. 2022. Temperature requirements of Colletotrichum spp. belonging to different clades. Frontiers in Plant Science, 13: 953760.
Shahbandeh, M. 2023. Global fruit production in 2021, by selected variety (in million metric tons)*. Statista.
Sharma, G., A. K. Pinnaka and B. D. Shenoy. 2015. Resolving the Colletotrichum siamense species complex using ApMat marker. Fungal Diversity, 71: 247-64.
Sutton, B., J. Bailey and M. Jeger. 1992. Colletotrichum: Biology, Pathology and ControlCAB International. Wallingford, UK.
Sutton, T., H. Aldwinckle, A. Agnello and J. Walgenbach. 2014. Compendium of Apple and Pear Diseases and Pests. American Phytopathological Society Press.
Tan, Q., G. Schnabel, C. Chaisiri, L.-F. Yin, W.-X. Yin and C.-X. Luo. 2022. Colletotrichum species associated with peaches in China. Journal of Fungi, 8: 313.
Tareen, M. J., M. Ali, U. Farooq, A. Yasin and S. K. Yasar. 2020. Apple cluster feasibility and transformation study, in Ali mubarik Cluster development based agriculture transformation plan vision-2025. Planning Commission of Pakistan and CAB International.
Tariq, A., F. Naz, C. Rauf, G. Irshad, N. Abbasi and N. Khokhar. 2017. First report of anthracnose caused by Colletotrichum truncatum on bell pepper (Capsicum annuum) in Pakistan. Plant Disease, 101: 631-31.
Velho, A., M. Stadnik, L. Casanova, P. Mondino and S. Alaniz. 2014. First report of Colletotrichum nymphaeae causing apple bitter rot in southern Brazil. Plant Disease, 98: 567-67.
Weir, B., P. Johnston and U. Damm. 2012. The Colletotrichum gloeosporioides species complex. Studies in mycology, 73: 115-80.
Wenneker, M., K. Pham, E. Kerkhof and D. O. Harteveld. 2021. First report of preharvest fruit rot of ‘Pink Lady’apples caused by Colletotrichum fructicola in Italy. Plant Disease, 105: 1561.
White, T., T. Bruns, S. Lee and J. Taylor. 1990. PCR protocols: A guide to methods and applications. Academic Press: New York, USA.
Zhou, Y., W. Zhang, Y. Li, S. Ji, X. Li, K. D. Hyde, K. Zhang, A. J. Phillips, I. S. Manawasinghe and J. Yan. 2023. Identification and characterization of Colletotrichum species associated with cherry leaf spot disease in China. Plant Disease, 107: 500-13.
DOI: 10.33687/phytopath.013.02.5097
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