Ralstonia pseudosolanacearum Infecting Pumpkin (Cucurbita Maxima) and its Pathogenicity on Cucurbitaceae Family

Angsana Akarapisan, Athidtaya Kumvinit, Malee Thungrabeab, Siwaporn Thumdee, Wichai Kositratana

Abstract


Ralstonia solanacearum species complex (RSSC) is a devastating phytopathogen with wide host range in many economically important crops all over the world. Bacterial wilt on a pumpkin (Cucurbita maxima) caused by Ralstonia pseudosolanacearum was observed in Chiang Rai, Thailand. The symptoms that appeared were wilting, drooping of half leaves, and oozing from crosscut stems of these diseased plants. The R. pseudosolanacearum-specific 280 bp amplicon, and sequevar 17 confirmed by sequencing the hrpB gene. The hrpB gene have been shown to be key determinants for pathogenicity in the phytopathogenic bacterial wilt. The susceptibility of different Cucurbitaceae species to bacterial wilt were tested. Members of the Cucurbita, Cucumis, Citrullus, Momordica, and Lagenaria genera were susceptible to bacterial wilt, with disease incidence ranging from 30% to 100%. The susceptibility of different Cucurbitaceae species to bacterial wilt, which can provide valuable information for the plant breeding programs to the improvement of pathogen-targeted.


Keywords


Bacterial wilt; Biovar; Pumpkin; Phylotype; Ralstonia pseudosolanacearum; sequevar

References


Akarapisan, A., A. Kumvinit, C. Nontaswatsri, T. Puangkrit and W. Kositratana. 2021. Phylotype, sequevar and pathogenicity of Ralstonia solanaceaum species complex from Northern Thailand. Journal of Phytopathology, 170: 176-84.

Aldon, D., B. Brito, C. Boucher and S. Genin. 2000. A bacteriai sensor of plant cell contact controls the transcriptional induction of Ralstonia solanaceaum pathogenicity gene. The EMBO Journal, 19: 2304-14.

Cheng, H.-R. and N. Jiang. 2006. Extremely rapid extraction of DNA from bacteria and yeasts. Biotechnology Letters, 28: 55-59.

Delaspre, F., C. G. Nieto Peñalver, O. Saurel, P. Kiefer, E. Gras, A. Milon, C. Boucher, S. Genin and J. A. Vorholt. 2007. The Ralstonia solanacearum pathogenicity regulator HrpB induces 3-hydroxy-oxindole synthesis. Proceedings of the national academy of sciences, 104: 15870-75.

Dhital, S. P., N. Thaveechai and S. K. Shrestha. 2001. Characteristics of Ralstonia solanacearum strains of potato wilt disease from Nepal and Thailand. Nepal Agriculture Research Journal, 4: 42-47.

Fegan, M. and P. Prior. 2005. How complex is the Ralstonia solanacearum species complex. In, Bacterial wilt disease and the Ralstonia solanacearum species complex.

French, E., P. Aley, E. Torres and U. Nydegger. 1993. Diversity of Pseudomonas solanacearum in Peru and Brazil. Australian Centre for International Agricultural Research. Place Published. pp.70-70.

Genin, S. and C. Boucher. 2004. Lessons learned from the genome analysis of Ralstonia solanacearum. Annual Review of Phytopathology, 42: 107-34.

Genin, S. and T. P. Denny. 2012. Pathogenomics of the Ralstonia solanacearum species complex. Annual Review of Phytopathology, 50: 67-89.

Liu, Z. Y., L. F. Zou, X. B. Xue, L. L. Cai, W. X. Ma, L. Xiong, Z. Y. Ji and G. Y. Chen. 2014. HrcT is a key component of the Type III secretion system in Xanthomonas spp. and also regulates the expression of key hrp transcriptional activator HrpX. . Applied Environment Microbiology, 80: 3908.

Opina, N., F. Tavner, G. Hollway, J. Wang, T. Li, R. Maghirang, M. Fegan, A. Hayward, V. Krishnapillai and W. Hong. 1997. A novel method for development of species and strain-specific DNA proves and PCR primers for identifying Burkholderia solanacearum (formerly Pseudomonas solanacearum). Asia Pacific Journal of Molecular Biology and Biotechnology, 5: 19-30.

Poussier, S., P. Prior, J. Luisetti, C. Hayward and M. Fegan. 2000. Partial sequencing of the hrpB and endoglucanase genes confirms and expands the known diversity within the Ralstonia solanacearum species complex. Systematic and Applied Microbiology, 23: 479–86.

Poussier, S., P. Vandewalle and J. Luisetti. 1999. Genetic diversity of African and worldwide strains of Ralstonia solanacearum as determined by PCR-restriction fragment length polymorphism analysis of the hrp gene region. Applied and Environmental Microbiology, 65: 2184-94.

Prior, P., F. Ailloud, B. L. Dalsing, B. Remenant, B. Sanchez and C. Allen. 2016. Genomic and proteomic evidence supporting the division of the plant pathogen Ralstonia solanacearum into three species. BMC Genomics, 17: 1-11.

Safni, I., I. Cleenwerck, P. De Vos, M. Fegan, L. Sly and U. Kappler. 2014. Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: Proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotype I and III strains as Ralstonia pseudosolanacearum sp. nov. International Journal of Systematic and Evolutionary Microbiology, 64: 3087-103.

She, X., L. Yu, G. Lan, Y. Tang and Z. He. 2017. Identification and genetic characterization of Ralstonia solanacearum species complex isolates from Cucurbita maxima in China. Frontiers in Plant Science, 8: 1794.

Thammakijjawat, P., N. Thaveechai, W. Kositratana, J. Chunwongse, R. D. Frederick and N. W. Schaad. 2001. Genetic analysis of Ralstonia solanacearum strains from different hosts in Thailand using PCR-restriction fragment length polymorphism. Agriculture and Natural Resources, 35: 397-408.

Thano, P. and A. Akarapisan. 2018. Phylotype and sequevar of Ralstonia solanacearum which causes bacterial wilt in Curcuma alismatifolia Gagnep. Letters in Applied Microbiology, 66: 384-93.

Vasse, J., S. Genin, P. Frey, C. Boucher and B. Brito. 2000. The hrpB and hrpG regulatory genes of Ralstonia solanacearum are required for different stages of the tomato root infection process. Molecular plant-microbe interactions, 13: 259-67.

Xu, J., Z. Pan, P. Prior, J. Xu, Z. Zhang, H. Zhang, L. Zhang, L. He and J. Feng. 2009. Genetic diversity of Ralstonia solanacearum strains from China. European Journal of Plant Pathology, 125: 641-53.

Yang, W., Q. Xu, H.-X. Liu, Y.-P. Wang, Y.-M. Wang, H.-T. Yang and J.-H. Guo. 2012. Evaluation of biological control agents against Ralstonia wilt on ginger. Biological Control, 62: 144-51.


Full Text: PDF

DOI: 10.33687/phytopath.013.02.4763

Refbacks

  • There are currently no refbacks.




Copyright (c) 2024 Angsana Akarapisan, Athidtaya Kumvinit, Malee Thungrabeab, Siwaporn Thumdee, Wichai Kositratana

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.