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  2. Vol 11, No 3 (2022)
  3. Mahmood

Alteration in Biochemical Responses in Leaves of Potato due to Common Scab Disease

Kamra Mahmood, Shahbaz T. Sahi, Muhammad Atiq, Muhammad Shahid, Sohail Ahmed

Abstract


The current study was designed at the research area of Plant Pathology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan during 2017– 2020. Fresh leaves of inoculated and un-inoculated potato varieties highly susceptible (FD 76-36), moderately susceptible (FD 73-110), and resistant (Esmee)/ test lines were collected during 2017-18 and 2018-19, at 35, 65 and 95 days after sowing for biochemical analysis. Superoxide dismutase activity, Catalase activity, Per-oxidase activity, Protein and Total phenolics contents were quantified. FD 76-36 exhibited minimum SOD activity (67.733, 45.637, 24.910) %, followed by FD 73-110 (70.303,57.893, 42.513) % and Esmee (84.567, 65.167, 45.873) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum CAT activity (10.990, 7.473, 3.413) %, followed by Esmee (13.537, 9.630, 8.147) % and FD 73-110 (19.277, 8.147, 6.170) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 exhibited minimum POD activity (0.2133, 0.2767, 0.3600) %, followed by FD 73-110 (0.2900, 0.5567, 0.6300) % and Esmee (0.8800, 1.2733, 1.5433) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum protein contents (3.097, 2.873, 1.260) %, followed by FD 73-110 (7.907, 5.423, 4.267) % and Esmee (12.163, 8.633, 5.127) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum TPC (136.31, 115.58, 70.77) %, followed by FD 73-110 (165.85, 136.75, 86.89) % and Esmee (188.42, 158.38, 109.00) % after 35, 65 and 95 days of inoculation respectively as compared to control. The experiment was conducted with a randomized complete block design (RCBD) and three replications. The experimental data were analyzed using Fisher’s analysis of variance technique and treatment means were compared by the least significance difference (LSD) test at a 5% probability level

Keywords


Solanum tuberosum; Streptomyces scabies; Enzymatic Metabolities; Non- Enzymatic Metabolites

References


Al-Mughrabi, K. I., A. Vikram, R. Poirier, K. Jayasuriya and G. Moreau. 2016. Management of common scab of potato in the field using biopesticides, fungicides, soil additives, or soil fumigants. Biocontrol Science and Technology, 26: 125-35. https://doi.org/10.1080/09583157.2015.1079809

Bhardwaj, R., N. Handa, R. Sharma, H. Kaur, S. Kohli, V. Kumar and P. Kaur. 2014. Lignins and abiotic stress: An overview. In, Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment. Springer. New York, USA. https://doi.org/10.1007/978-1-4614-8591-9_10

Boriboonkaset, T., C. Theerawitaya, N. Yamada, A. Pichakum, K. Supaibulwatana, S. Cha-Um, T. Takabe and C. Kirdmanee. 2013. Regulation of some carbohydrate metabolism-related genes, starch and soluble sugar contents, photosynthetic activities and yield attributes of two contrasting rice genotypes subjected to salt stress. Protoplasma, 250: 1157-67. https://doi.org/10.1007/s00709-013-0496-9

Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72: 248-54. https://doi.org/10.1016/0003-2697(76)90527-3

Carmen, N. 1995. Seed bacteriology laboratory guide Seed Pathology. Danish Government Institute.

Cernadas, R. A., L. R. Camillo and C. E. Benedetti. 2008. Transcriptional analysis of the sweet orange interaction with the citrus canker pathogens Xanthomonas axonopodis pv. citri and Xanthomonas axonopodis pv. aurantifolii. Molecular plant pathology, 9: 609-31. https://doi.org/10.1111/j.1364-3703.2008.00486.x

Fu, J., B. Huang and G. Zhang. 2000. Physiological and biochemical changes during seed filling in relation to leaf senescence in soybean. Biologia Plantarum, 43: 545-48. https://doi.org/10.1023/A:1002857328923

Gomes, M. P., E. Smedbol, A. Chalifour, L. Hénault-Ethier, M. Labrecque, L. Lepage, M. Lucotte and P. Juneau. 2014. Alteration of plant physiology by glyphosate and its by-product aminomethylphosphonic acid: An overview. Journal of experimental botany, 65: 4691-703. https://doi.org/10.1093/jxb/eru269

Goszczynska, T., J. Serfontein and S. Serfontein. 2000. Introduction to Practical Phytobacteriology:A Laboratory Manual for Phytobacteriology. ARCPPRI Pretoria: South Africa.

Goth, R. W., K. Haynes, R. Young, D. Wilson and F. Lauer. 1995. Relative resistance of the potato cultivar Krantz to common scab caused by Streptomyces scabies as determined by cluster analysis. American Potato Journal, 72: 505-11. https://doi.org/10.1007/BF02849252

Hameed, A., M. Atiq, S. T. Sahi, N. A. Rajput, Z. Ahmed, M. W. Alam, H. Alsamadany, Y. Alzahrani, S. Sarfraz and J. Altaf. 2021. Biochemical base of resistance in citrus against canker disease. PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES, 58: 1850-58. https://doi.org/10.21162/PAKJAS/21.11

Iriti, M. and E. M. Varoni. 2015. Chitosan-induced antiviral activity and innate immunity in plants. Environmental Science and Pollution Research, 22: 2935-44. https://doi.org/10.1007/s11356-014-3571-7

Khan, M. M., K. N. Bashir and M. A. Ali. 1999. Occurrence of common scab of potato in different potato growing area of Pakistan. Sarhad Journal of Agriculture, 11: 33-37.

Kumar, N., R. C. Ebel and P. D. Roberts. 2011. H2O2 degradation is suppressed in kumquat leaves infected with Xanthomonas axonopodis pv. citri. Scientia Horticulturae, 130: 241-47. https://doi.org/10.1016/j.scienta.2011.07.005

Lattanzio, V., V. M. Lattanzio and A. Cardinali. 2006. Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochemistry: Advances in research, 661: 23-67.

Lerat, S., A. M. Simo‐Beaunoir and C. Beaulieu. 2009. Genetic and physiological determinants of Streptomyces scabies pathogenicity. Molecular plant pathology, 10: 579-85. https://doi.org/10.1111/j.1364-3703.2009.00561.x

Li, C., W. Shi, D. Wu, R. Tian, B. Wang, R. Lin, B. Zhou and Z. Gao. 2021. Biocontrol of potato common scab by Brevibacillus laterosporus BL12 is related to the reduction of pathogen and changes in soil bacterial community. Biological Control, 153: 104496. https://doi.org/10.1016/j.biocontrol.2020.104496

Liu, D., J. Zou, Q. Meng, J. Zou and W. Jiang. 2009. Uptake and accumulation and oxidative stress in garlic (Allium sativum L.) under lead phytotoxicity. Ecotoxicology, 18: 134-43. https://doi.org/10.1007/s10646-008-0266-1

Mikulic‐Petkovsek, M., V. Schmitzer, F. Stampar, R. Veberic and D. Koron. 2014. Changes in phenolic content induced by infection with Didymella applanata and Leptosphaeria coniothyrium, the causal agents of raspberry spur and cane blight. Plant pathology, 63: 185-92. https://doi.org/10.1111/ppa.12081

Naqvi, S. A., M. Khan, M. Shahid, M. Jaskani, I. A. Khan, M. Zuber and K. M. Zia. 2011. Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks. Carbohydrate polymers, 83: 623-28. https://doi.org/10.1016/j.carbpol.2010.08.031

O'Brien, J. A., A. Daudi, P. Finch, V. S. Butt, J. P. Whitelegge, P. Souda, F. M. Ausubel and G. P. Bolwell. 2012. A peroxidase-dependent apoplastic oxidative burst in cultured Arabidopsis cells functions in MAMP-elicited defense. Plant Physiology, 158: 2013-27. https://doi.org/10.1104/pp.111.190140

Pandey, S., D. Fartyal, A. Agarwal, T. Shukla, D. James, T. Kaul, Y. K. Negi, S. Arora and M. K. Reddy. 2017. Abiotic stress tolerance in plants: myriad roles of ascorbate peroxidase. Frontiers in plant science, 8: 581. https://doi.org/10.3389/fpls.2017.00581

Ramzan, M., S. Sana, N. Javaid, A. A. Shah, S. Ejaz, W. N. Malik, N. A. Yasin, S. Alamri, M. H. Siddiqui and R. Datta. 2021. Mitigation of bacterial spot disease induced biotic stress in Capsicum annuum L. cultivars via antioxidant enzymes and isoforms. Scientific Reports, 11: 9445. https://doi.org/10.1038/s41598-021-88797-1

Reddy, B., R. Mandal, M. Chakroborty, L. Hijam and P. Dutta. 2018. A review on potato (Solanum tuberosum L.) and its genetic diversity. International Journal of Genetics, 10: 360-64. https://doi.org/10.9735/0975-2862.10.2.360-364

Shahid, M., M. Khan, A. Hameed, M. Ashraf and A. Jamil. 2012. Antioxidant enzymes and inorganic elements in seeds and leaves of four potential medicinal plants from Pakistan. AGROCHIMICA, 56: 281-91.

Siddique, Z., K. P. Akhtar, A. Hameed, N. Sarwar, Imran-Ul-Haq and S. A. Khan. 2014. Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virus. Journal of Plant Interactions, 9: 702-11. https://doi.org/10.1080/17429145.2014.905800

Steel, R. G. D., J. H. Torrie and D. A. Dickey. 1997. Principles and Procedures of Statistics. McGraw Hill Book Co.: New York.

Turkensteen, L. J. 1986. Survey on bacterial and fungal potato diseases in hilly areas of Pakistan August-September 1985PSPDP. Islamabad, Pakistan.

Zhang, Y., Y. Ikeno, W. Qi, A. Chaudhuri, Y. Li, A. Bokov, S. R. Thorpe, J. W. Baynes, C. Epstein and A. Richardson. 2009. Mice deficient in both Mn superoxide dismutase and glutathione peroxidase-1 have increased oxidative damage and a greater incidence of pathology but no reduction in longevity. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, 64: 1212-20. https://doi.org/10.1093/gerona/glp132


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DOI: 10.33687/phytopath.011.03.4445

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Copyright (c) 2023 Kamra Mehmood, Shahbaz Talib Sahi, Muhammad Atiq, Muhammad Shahid

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