Detection of Resistance against Leaf Spot, Yellow Mosaic and Urdbean Leaf Crinkle Viruses in Mash Bean (Vigna mungo)

Manzoor Hussain, Amir Afzal, Muhammad Zeeshan, Sairah Syed, Tamoor Hussain, Ghulam Nabi, Bashir Ahmad, Muhammad Ijaz


Among foliar diseases Leaf spot, yellow mosaic virus (YMV) and Urd bean leaf crinkle virus (ULCV) are destructive diseases of mash bean (Vigna mungo) in Pakistan. Incorporation of resistance against biotic stress in plants is traditional strategy of crop improvement through breeding. Effective breeding for biotic stresses entails readily accessible resistant germplasm. 67 genotypes were evaluated for multi disease resistance was tested for two consecutive years (2019 and 2020) at Barani Agricultural Research Institute, Chakwal. Experiment was repeated with an idea to decrease the effect of environment and those material was selected which performed same in both years. Out of 67 genotypes 12 were resistant against leaf spot infection and 19 genotypes were found highly resistant against yellow mosaic virus during both years. In case of ULCV disease did not appear during 2020. However, 19 genotypes showed resistance against ULCV during first year of experiment. Two genotypes namely 3CM-703 and 4CM-720 exhibited resistance response to all foliar diseases under study. Three genotypes viz. 4CM-716, 4CM-720, 5CM-708 demonstrated resistance against YMV as well as leaf spot. 4CM716, 4CM720, 5CM708, 7CM701, 7CM702, 7CM704, 7CM705, 7CM706 expressed resistance against leaf spot and ULCV simultaneously. The data generated is useful for improvement of mash bean through breeding.


Mash bean; Vigna mungo (L.); Leaf spot; YMY; ULCV; disease resistance


Achakzai, A. K. K. and S. A. Taran. 2011. Effect of seed rate on growth, yield components and yield of mash bean grown under irrigated conditions of arid uplands of Balochistan, Pakistan. Pakistan Journal of Botany, 43: 961-69.

Aftab, M., S. Iqbal, S. Khalid and M. Bashir. 1993. Urdbean leaf crinkle virus (ULCV) and its effect on yield and growth components of genotypes. 4th National Conference Plant Scientists, Faisalabad, Pakistan.

Agrios, G. 2005. Plant Pathology. Elsevier Academic Press: Burlington, MA. USA.

Ahmad, M. 1975. Screening of mungbean (Vigna radiata) and urdbean (V. mungo) germplasms for resistance to yellow mosaic virus. Journal of Agricultural Research, 13: 349-54.

Ahmad, M. and R. Harwood. 1973. Studies on whitefly transmitted yellow mosaic of urdbeans (Phaseolus mungo). Plant Disease Reporter, 57: 800-02.

Ahmad, Z., M. Bashir and T. Mtsueda. 1997. Evaluation of legume germplasm for seed borne viruses: Harmonizing Agricultural productivity and conservation of biodiversity. Proceeding of 8th SABRAO General Congress and Annual Meeting of Korean Breeding Society, Seoul, Korea.

Ahmed, Z. I., M. Ansar, A. Saleem, Z. ullah Arif, H. Javed and R. Saleem. 2012. Improvement of mash bean production under rainfed conditions by rhizobium inoculation and low rates of starter nitrogen. Pakistan Journal of Agricultural Research, 25: 154-60.

Ali, A. and M. Akram. 1999. Mash-97: The first short duration and short stature variety of black gram (Vigna mungo (L) Hopper). Journal of Agricultural Research, 37: 31-37.

Ashfaq, M., M. A. Khan, S. Mughal, N. Javed, T. Mukhtar and M. Bashir. 2007. Evaluation of urdbean germplasm for resistance against urdbean leaf crinkle virus. Pakistan Journal of Botany, 39: 2103-11.

Bakar, A. 1981. Pest and disease problem in mungbean in west Malaysia. Malaysian Agricultural Journal, 53.

Bashir, M. 2005. Studies on viral diseases of major pulse crops and identification of resistant sources. Technical Annual Report of ALP Project. National Agricultural. Research Centre. Islamabad, Pakistan, pp. 1-169.

Bashir, M., Z. Ahmad and T. Iqbal. 1991. Detection and Identification of two seed borne potyviruses from imported seeds of cowpea (Vigna unguiculata) from Nigeria. Proceedings of Second National Conference of Plant Pathology, University of Agriculture, Faisalabad, Pakistan.

Bashir, M., Z. Ahmad and S. Mansoor. 2006. Occurrence and distribution of viral diseases of mungbean and mashbean in Punjab, Pakistan. Pakistan Journal of Botany, 38: 1341-51.

Bashir, M., A. Ghafoor, M. Zubair and B. Malik. 1996. Screening of mash germplasm and advanced breeding lines for virus diseases under field conditions. Crop Protection Conference NWFP Agriculture University, Peshawar, Pakistan.

Bashir, M. and B. A. Malik. 1988. Diseases of major pulse crops in Pakistan: A review. International journal of pest management, 34: 309-14.

Bashir, M., A. Zahoor and N. Murata. 2000. Seed bone Viruses: Detection, Identification and Control. National Agriculture Reserach Center. Islamabad, Pakistan.

Bashir, M. and M. Zubair. 2002. Identification of resistance in urdbean (Vigna mungo) against two different viral diseases. Pakistan Journal of Botany, 34: 49-51.

Beniwal, S., S. Kolte and Y. Nene. 1980. Nature and spread of urdbean leaf crinkle virus under field conditions. Indian Journal of Mycology and Plant Pathology, 9: 188-92.

Bhanu, A. N., M. Singh and K. Srivastava. 2017. Screening mungbean [Vigna radiata (L.) Wilczek] genotypes for mungbean yellow mosaic virus resistance under natural condition. Advances in Plants and Agricultural Research, 7: 417-20.

Binyamin, R., M. A. Khan, A. Khan, M. A. Khan, F. Awan and N. Khan. 2011. Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus. Genetics and Molecular Research, 10: 1681-88.

FAO. 2016. Food and agriculture organization of the United Nations. Food and Agriculture Organization. Rome, Italy.

Ghafoor, A., C. Rauf and S. Iqbal. 1996. Screening for resistance in mash against yellow mosaic disease. Proceedings of Crop Protection Conference, Peshawar. Pakistan.

Gong, F., L. Yang, F. Tai, X. Hu and W. Wang. 2014. “Omics” of maize stress response for sustainable food production: opportunities and challenges. Omics: a journal of integrative biology, 18: 714-32.

GOP. 2017. Crop Area and Production 2015-16 & 2016-17. Ministry of Food, and Agriculture (Economic Wing). Islamabad, Pakistan.

GOP. 2019. Crop Area and Production 2018-19. Ministry of Food, and Agriculture (Economic Wing). Islamabad, Pakistan.

Haq, A. 1991. Screening of Urdbean germplasm against Urdbean leaf crinkle virus and yellow mosaic virus and quantitative determination of growth responses of Urdbean cultivars against virus infection, University of Agriculture Faisalabad.

Iqbal, S., A. Ghafoor, M. Zubair and B. Malik. 1991. Reaction of urdbean cultivars against leaf crinkle virus disease. Journal of Agricultural Research, 29: 411-15.

Iqbal, S., M. Zubair and A. Ghafoor. 1990. Screening of mash against yellow mosaic disease. Sarhad Journal of Agriculture, 6: 403-05.

Jayasakera, S. and H. Ariyarantne. 1988. Current status of mungbean improvement for farming system in Sri Lanka. Mungbean proceedings of the Second International Symposium, Bangkok, Thailand.

Kadian, O. 1980. Studies on leaf crinkle disease of Urdbean (Vigna mungo (L.) Hepper) mungbean Vigna radiata (L) Wilszek and its control, Haryana Agriculture University.

Kolte, S. and Y. Nene. 1972. Studies on symptoms and mode of transmission of the leaf crinkle virus of urd bean (Phaseolus mungo). Indian Phytopathology, 25: 401-04.

Malik, I. 1991. Breeding for resistance to MYMV and its vector in Pakistan. Mungbean Yellow Mosaic Disease. Proceedings of An International Workshop, Bangkok, Thailand.

Malik, I., Y. Ali and M. Saleem. 1988. Incorporation of tolerance to Mungbean Yellow Mosaic virus from local germplasm to exotic large seeded mungbean. Mungbean proceedings of the Second International Symposium, Bangkok, Thailand.

Mayee, C. and V. Datar. 1986. Phytopathometery Technical Bulletin 1Marathwada Agricultral University. Parbhani, India.

Munawwar, M. H., A. Ali and S. R. Malik. 2014. Identification of resistance in mungbean and mashbean germplasm against mungbean yellow mosaic virus. Pakistan Journal of Agricultural Research, 27: 129-35.

Nene, Y. 1972. A survey of viral diseases of Pulse crops in Uttar Pradesh. Research Bulletin-4Govind Ballabh. Pant University of Agriculture and Technology. Pant Nagar, India. pp. 191.

Qayyum, A., J. Iqbal, L. Barbanti, A. Sher, G. Shabbir, G. Rabbani, M. Rafiq, M. Tareen, M. Tareen and B. Amin. 2019. Mash bean [Vigna mungo (L.) Hepper] germplasm evaluation at different ecological conditions of Pakistan. Applied Ecology and Environmental Research, 17: 6643-54.

Riaz, Z., T. Mitsueda, M. Bashir and Z. Ahmad. 1994. Occurrence of seed-borne pathogens in germplasm collection. National Agricultural Research Center. Islamabad. Pakistan, pp. 48-54.

Rishi, N. 1990. Seed and crop improvement of northern Indian pulses (Pisum & Vigna) through control of seed mosaic virus. Final Technical Report Department of Plant Pathology, Haryana Agricultural University. Hissar. India.

Sarwar, G. and M. Rajput. 1999. Role of nuclear technology in development of new high yielding mungbean varieties. Proceedings of Genetical approaches to Crop improvement, Plant Genetic Division, Tandojam Pakistan.

Shakoor, A., M. Haq, M. Sadiq and M. Sarwar. 1977. Induction of resistance to Yellow Mosaic virus in mungbean through induced mutation. Plant Disease, 14: 293-302.

Verdcourt, B. 1970. Studies in the leguminosae-papilionideae for the flora of tropical east AfricaKew Bulletin.

Zahid, M. 2011. Identification of resistant mungbean and mash bean genotypes against Cercospora leaf spot for incorporating in breeding programme to develop disease resistant varieties. Pakistan Agriculture Reserach Council. Islamabad, Pakistan.

Full Text: PDF

DOI: 10.33687/phytopath.011.02.4274


  • There are currently no refbacks.

Copyright (c) 2022 Amir Afzal

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.