Management of Late Blight of Potato caused by Phytophthora infestans through Botanical Aqueous Extracts

Basharat Mehmood, Aneela Azad, Nasir Rahim, Shameen Arif, Muhammad R. Khan, Abid Hussain, Muhammad Tariq-Khan, Muhammad T. Younis, Asma Bashir, Sohail Ahmed, Javed R. Khan, Sahira Tariq, Muhammad Jamil


Late blight caused by Phytophthora infestans (Mont.) de Bary is most devastating pathogen of potato crop. Disease caused Ireland's worst devastation during 1840–1845 and since then, it has caused considerable yield losses globally. The repeated and injudicious synthetic fungicide applications against late blight developed fungicide resistance in P. infestans have given a push to finding out alternate ways to control the disease. The study was carried out to evaluate the efficacy of garlic (Allium sativum), neem (Azadirachta indica), turmeric (Curcuma longa), mint (Mentha) at 5, 10 and 15% concentrations as bio-fungicides against late blight of potato. In-vitro effect of aqueous plant extracts was evaluated on percent inhibition and radial growth of pathogen. In comparison to the control, A. sativum and A. indica at 15% concentration were found to be more effective in inhibiting P. infestans mycelial growth by 58.4% and 43.9%, respectively. In the greenhouse trials, overall potato late blight disease incidence was minimum (5.81%) where A. sativum extract was used followed by A. indica (8.45%) at 15% concentration as compared to control (61.18%). Similarly, 15% aqueous extracts of A. sativum was found highly effective with 15.4% disease severity, as compared to control (54.13%). The use of A. sativum and A. indica aqueous plant extracts at a concentration of 30% was found to be the most promising and effective measure against the late blight pathogen. Study provides a comparison of environment friendly botanical extract based disease control for organic vegetable production opportunity inspite of health hazardous synthetic agro-chemicals.


Garlic; Late blight; Neem; Potato; Plant extracts; Turmeric


Adl, S. M., A. G. Simpson, M. A. Farmer, R. A. Andersen, O. R. Anderson, J. R. Barta, S. S. Bowser, G. Brugerolle, R. A. Fensome and S. Fredericq. 2005. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. Journal of Eukaryotic Microbiology, 52: 399-451.

Alhoot, M. A., M. Eltraiki and P. Ridzuan. 2019. The inhibitory effect of the Mentha piperita leaves extracts on the mycotoxin producer Aspergillus niger. International Journal of Medical Toxicology and Legal Medicine, 22: 179-83.

Aslam, A., F. Naz, M. Arshad, R. Qureshi and C. Rauf. 2010. In vitro antifungal activity of selected medicinal plant diffusates against Alternaria solani, Rhizoctonia solani and Macrophomina phaseolina. Pakistan Journal of Botany, 42: 2911-19.

Bashir, A., M. T. Khan, R. Ahmed, B. Mehmood, M. T. Younas, H. M. Rehman and S. Hussain. 2020. Efficiency of selected botanicals against (Alternaria solani) causing early blight disease on tomato in Azad Jammu and Kashmir. Pakistan Journal of Phytopathology, 32: 179-86.

Berhan, M. 2021. Review on epidemiology, sampling techniques, management strategies of late blight (Phytophthora infestans) of potato and its yield loss. Asian Journal of Advances in Research, 7: 9-17.

Bianchi, A., A. Zambonelli, A. Z. D'Aulerio and F. Bellesia. 1997. Ultrastructural studies of the effects of Allium sativum on phytopathogenic fungi in vitro. Plant disease, 81: 1241-46.

Dahlin, P., V. Srivastava, S. Ekengren, L. S. McKee and V. Bulone. 2017. Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans. PLoS One, 12: e0170873.

Derbalah, A., M. El-Mahrouk and A. El-Sayed. 2011. Efficacy and safety of some plant extracts against tomato early blight disease caused by Alternaria solani. Plant Pathology Journal, 10: 115-21.

FAO. 2020. Statistical report on mango production in Pakistan. FAO Statistical Division. Place Published.

Fontem, D. A., O. M. Olanya, G. R. Tsopmbeng and M. A. P. Owona. 2005. Pathogenicity and metalaxyl sensitivity of Phytophthora infestans isolates obtained from garden huckleberry, potato and tomato in Cameroon. Crop Protection, 24: 449-56.

França, K., T. Silva, T. Cardoso, A. Ugulino, A. Rodrigues and A. de Mendonça Júnior. 2018. In vitro effect of essential oil of peppermint (Mentha piperita L.) on the mycelial growth of Alternaria alternata. Journal of Experimental Agriculture International, 26: 1-7.

Ghazanfar, M. U., S. T. Sahi, W. Wakil and Z. Iqbal. 2010. Evaluation of various fungicides for the management of late blight of potato (Phytophthora infestans). Pakistan Journal of Phytopathology, 22: 83-88.

Ghorbani, R., S. Wilcockson and C. Leifert. 2005. Alternative treatments for late blight control in organic potato: Antagonistic micro-organisms and compost extracts for activity against Phytophthora infestans. Potato Research, 48: 181-89.

Gopi, R., R. Avasthe, H. Kalita, A. Yadav, S. Das and D. Rai. 2020. Eco-friendly management of tomato late blight using botanicals, bio-control agents, compost tea and copper fungicides.

Gurjar, M. S., S. Ali, M. Akhtar and K. S. Singh. 2012. Efficacy of plant extracts in plant disease management. Agricultural Sciences, 3: 425-33.

Halama, P. and C. Van Haluwin. 2004. Antifungal activity of lichen extracts and lichenic acids. Biological Control, 49: 95-107.

Hyder, S., A. S. Gondal, Z. F. Rizvi, R. Ahmad, M. M. Alam, A. Hannan, W. Ahmed, N. Fatima and M. Inam-ul-Haq. 2020. Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Scientific reports, 10: 1-15.

Kagale, S., T. Marimuthu, B. Thayumanavan, R. Nandakumar and R. Samiyappan. 2004. Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiological and Molecular Plant Pathology, 65: 91-100.

Kato, M., E. S. Mizubuti, S. B. Goodwin and W. E. Fry. 1997. Sensitivity to protectant fungicides and pathogenic fitness of clonal lineages of Phytophthora infestans in the United States. Phytopathology, 87: 973-78.

Krebs, H., B. Dorn and H.-R. Forrer. 2006. Control of late blight of potato with medicinal plant suspensions. Agrarforschung, 13: 16-21.

Majeed, A. and Z. Muhammad. 2018. Potato production in Pakistan: challenges and prospective management strategies-a review. Pakistan Journal of Botany, 50: 2077-84.

Moumene, M., F. Ohbunmi and D. Saddek. 2015. In Vitro and In Vivo effects of aqueous extract of Rosmarinus officinalis L. (Rosemary) in the control of late blight disease of potato Caused by Phytophthora Infestans. Advances In Crop Science and Technology, 3: 2329- 8863.

Olanya, O. and R. Larkin. 2006. Efficacy of essential oils and biopesticides on Phytophthora infestans suppression in laboratory and growth chamber studies. Biocontrol Science and Technology, 16: 901-17.

Paranagama, P., K. Abeysekera, K. Abeywickrama and L. Nugaliyadde. 2003. Fungicidal and anti‐aflatoxigenic effects of the essential oil of Cymbopogon citratus (DC) Stapf (lemongrass) against Aspergillus flavus Link isolated from stored rice. Letters in Applied Microbiology, 37: 86-90.

Poehlm, J. M. and D. A. Slepper. 1995. Breeding Field Crops. 4th Ed. Iowa State Univ. Press. Place Published.

Raza, W., M. U. Ghazanfar and M. I. Hamid. 2019. Occurrence of late blight (Phytophthora infestans (Mont) De Bary) in major potato growing areas of Punjab, Pakistan. Sarhad J. Agric, 35: 806-15.

Rogozhin, E. A., A. S. Vasilchenko, A. S. Barashkova, A. N. Smirnov, S. K. Zavriev and V. P. Demushkin. 2020. Peptide extracts from seven medicinal plants discovered to inhibit oomycete phytophthora infestans, a causative agent of potato late blight disease. Plants, 9: 1294.

Sajid, Z. A. and F. Aftab. 2012. Role of salicylic acid in amelioration of salt tolerance in potato (Solanum tuberosum L.) under in vitro conditions. Pakistan Journal of Botany, 44: 37-42.

Saleem, M. Y. Khalid Pervaiz Akhtar, Qumer Iqbal, Muhammad Asghar, Amjad Hameed and Muhammad Shoaib. 2016. Development of tomato hybrids with multiple disease tolerance. Pakistan Journal of Botany, 48(2): 771-778

Sallam, M. and K. A. Abo-ElyouSr. 2013. Evaluation of various plant extracts against the early blight disease of tomato plants under greenhouse and field conditions. Plant Protection Science, 48: 74-79.

Shattock, R. C. 2002. Phytophthora infestans: populations, pathogenicity and phenylamides. Pest Management Science, 58: 944-50.

Spooner, D. M., K. McLean, G. Ramsay, R. Waugh and G. J. Bryan. 2005. A single domestication for potato based on multilocus amplified fragment length polymorphism genotyping. Proceedings of the National Academy of Sciences, 102: 14694-99.

Stephan, D., A. Schmitt, S. M. Carvalho, B. Seddon and E. Koch. 2005. Evaluation of biocontrol preparations and plant extracts for the control of Phytophthora infestans on potato leaves. European Journal of Plant Pathology, 112: 235-46.

Taskeen-un-Nisa, W. A. and R. Mir. 2010. Antimycotic activity of plant extracts on the spore germination of some pathogenic fungi. Mycopath, 8: 65-69.

Thomas, L. M. and B. G. Naik. 2017. Evaluation of Different Culture Media, Fungicides and Bio Control Agents on the Growth of Phytopthora Capsici Leonian. Causing Foot Rot of Black Pepper in Vitro. Chemical Science Review and Letters, 2017: 279-86.

Wang, W., B. Ben-Daniel and Y. Cohen. 2004. Control of plant diseases by extracts of Inula viscosa. Phytopathology, 94: 1042-47.

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DOI: 10.33687/phytopath.011.01.4131


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