Antagonizing Impact of Endophytic Fungal Isolates against Potato Black Scurf (Rhizoctonia solani)

Boutheina Mejdoub-Trabelsi, Rania Aydi-Ben Abdallah, Hayfa Jabnoun-Khiareddine, Ayed Faker, Mejda Daami-Remadi


Four endophytic fungal isolates, naturally associated with Solanum tuberosum L., were evaluated for their antagonistic activity against Rhizoctonia solani and their ability to suppress black scurf disease of potato tubers. To identify the potential implication of antifungal compounds in their inhibitory activity, cell-free culture filtrates were produced and tested for their antifungal potential against R. solani mycelial growth and for their protective effect against development of on potato tuber slices.  Results showed that percent inhibition of R. solani mycelial growth, based on in vitro antibiosis tests, ranged between 16 to 59%. Hyphal damage and lysis were the most frequent stress responses exhibited by the target pathogen during its in vitro interactions with the potato-associated fungi tested. All Cell-free culture filtrates of tested fungi had significantly inhibited the radial growth of Rhizoctonia solani Rs20. The cell-free culture filtrates of Penicillium chrysogenum and Aspergillus niger had decreased pathogen growth by more than 60% over control. These two filtrates (P. chrysogenum and A. niger) were found to be the most effective in decreasing the decay incidence in potato slices by 36 and 40%, respectively, as compared to pathogen-inoculated and untreated control. Thus, the present study clearly demonstrated that fungal isolates, occurring ubiquitously within potato plants, may be explored as potent biocontrol agents against potato-associated fungal pathogens and as source of bioactive metabolites for R. solani suppression.


Aspergillus spp.; Biocontrol; Extracellular metabolites; Endophytic fungi; Penicillium spp.; Potato Black scurf


Abbas, H. K., R. M. Zablotowicz, H. A. Bruns and C. A. Abel. 2006. Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolates. Biocontrol Science and Technology, 16: 437-449.

Aydi Ben Abdallah, R., B. Mejdoub-Trabelsi, A. Nefzi, H. Jabnoun-Khiareddine and M. Daami-Remadi. 2016. Isolation of endophytic bacteria from Withania somnifera and assessment of their ability to suppress Fusarium wilt disease in tomato and to promote plant growth. Journal of Plant Pathology and Microbiology, 7: 2-11.

Aydi, R., M. Hassine, H. Jabnoun-Khiareddine, H. Ben Jannet and M. Daami-Remadi. 2013. Valorisation des Aspergillus spp. comme agents de lutte biologique contre Pythium et optimisation de leur pouvoir antagoniste in vitro et in vivo. Tunisian Journal of Medicinal Plants and Natural Products, 9: 70-82.

Aydi, R., M. Hassine, H. Jabnoun-Khiareddine, H. Ben Jannet and M. Daami-Remadi. 2014. Study of the antifungal potential of Aspergillus spp. and their culture filtrates and organic extracts against Fusarium sambucinum. Tunisian Journal of Medicinal Plants and Natural Products, 11: 15-29.

Berg, G., C. Zachow, J. Lottmann, M. Götz, R. Costa and K. Smalla. 2005. Impact of plant species and site on rhizosphere-associated fungi antagonistic to Verticillium dahliae Kleb. Applied and Environmental Microbiology, 71: 4203-4213.

Collinge, D. B., H. J. Jørgensen, M. A. Latz, A. Manzotti, F. Ntana, E. C. Rojas Tayo and B. Jensen. 2019. Searching for novel fungal biological control agents for plant disease control among endophytes, In: T. Hodkinson, F. Doohan, M. Saunders & B. Murphy, (eds.), Endophytes for a Growing World. Cambridge University Press, United Kingdom, pp. 25.

Daami-Remadi, M., S. Zammouri and M. El Mahjoub. 2008a. Effect of the level of seed tuber infection by Rhizoctonia solani at planting on potato growth and disease severity. The African Journal of Plant Science and Biotechnology, 2: 34-38.

Daami-Remadi, M., S. Zammouri and M. El Mahjoub. 2008b. Relative susceptibility of nine potato (Solanum tuberosum L.) cultivars to artificial and natural infection by Rhizoctonia solani as measured by stem canker severity, black scurf and plant growth. The African Journal of Plant Science and Biotechnology, 2: 57-66.

Debbab, A., A. H. Aly and P. Proksch. 2013. Mangrove derived fungal endophytes- A chemical and biological perception. Fungal diversity, 61: 1-27.

Djébali, N. and T. Belhassen. 2010. Field study of the relative susceptibility of eleven potato (Solanum tuberosum L.) varieties and the efficacy of two fungicides against Rhizoctonia solani attack. Crop Protection, 29: 998-1002.

Elkahoui, S., N. Djébali, N. Yaich, S. Azaiez, M. Hammami, R. Essid and F. Limam. 2015. Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani. World Journal of Microbiology and Biotechnology, 31: 175-185.

Figueiredo, M. d. V. B., A. Bonifacio, A. C. Rodrigues, F. F. d. Araujo and N. P. Stamford. 2016. Beneficial microorganisms: Current challenge to increase crop performance, Bioformulations for Sustainable Agriculture. Springer, p. 53-70.

Grosch, R., F. Faltin, J. Lottmann, A. Kofoet and G. Berg. 2005. Effectiveness of 3 antagonistic bacterial isolates to control Rhizoctonia solani Kühn on lettuce and potato. Canadian Journal of Microbiology, 51: 345-353.

Grosch, R., K. Scherwinski, J. Lottmann and G. Berg. 2006. Fungal antagonists of the plant pathogen Rhizoctonia solani: Selection, control efficacy and influence on the indigenous microbial community. Mycological Research, 110: 1464-1474.

Hassine, M., R. Aydi-Ben Abdallah, H. Jabnoun-Khiareddine, H. Ben Jannet and M. Daami-Remadi. 2013. Effet des températures d'incubation et des méthodes de confrontation sur le pouvoir inhibiteur exercé par Penicillium sp. et Gliocladium spp. sur Botrytis cinerea. Tunisian Journal of Medicinal Plants and Natural Products, 9: 41-51.

Hyder, S., A. S. Gondal, Z. F. Rizvi, R. Ahmad, M. M. Alam, A. Hannan, W. Ahmed, N. Fatima and M. Inam-ul-Haq. 2020. Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Scientific Reports, 10: 1-15.

Keiser, A. 2008. Rhizoctonia solani-A fungal disease with multiple symptoms: Means of preventive and curative control. Potato Research for a Production of Quality. Information Day, Changins.

Khedher, S. B., O. Kilani-Feki, M. Dammak, H. Jabnoun-Khiareddine, M. Daami-Remadi and S. Tounsi. 2015. Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato. Comptes Rendus Biologies, 338: 784-792.

Khedher, S. B., B. Mejdoub-Trabelsi and S. Tounsi. 2021. Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth. Biological Control, 152: 104444.

Kumar, S., R. P. Aharwal, H. Shukla, R. Rajak and S. S. Sandhu. 2014. Endophytic fungi: As a source of antimicrobials bioactive compounds. World Journal of Pharmacy and Pharmaceutical, 3: 1179-1197.

Larena, I., P. Sabuquillo, P. Melgarejo and A. De Cal. 2003. Biocontrol of Fusarium and Verticillium wilt of tomato by Penicillium oxalicum under greenhouse and field conditions. Journal of Phytopathology, 151: 507-512.

Mahdi, T., I. Mohamed and S. Yagi. 2014. Endophytic fungal communities associated with ethno-medicinal plants from Sudan and their antimicrobial and antioxidant prospective. Journal of Forest Products and Industries, 3: 248-256.

Marwa, H., A.-B. A. Rania, J.-K. Hayfa and D.-R. Mejda. 2014. Pouvoir antifongique des Penicillium sp. et des Gliocladium spp. contre Alternaria solani in vitro et sur fruits de tomate. Tunisian Journal of Medicinal Plants and Natural Products, 12: 9-28.

Mejdoub-Trabelsi, B., R. Abdallah, N. Ammar, Z. Kthiri and W. Hamada. 2016a. Bio-suppression of Fusarium wilt disease in potato using nonpathogenic potatoassociated fungi. Journal of Plant Pathology and Microbiology, 7: 1000347.

Mejdoub-Trabelsi, B., R. Abdallah, Z. Kthiri, W. Hamada and M. Remadi. 2016b. Assessment of the antifungal activity of non-pathogenic potato-associated fungi toward Fusarium species causing tuber dry rot disease. Journal of Plant Pathology and Microbiology, 7: 1000343.

Mrabet, M., N. Djebali, S. Elkahoui, Y. Miloud, S. Saidi, B. Tarhouni and R. Mhamdi. 2013. Efficacy of selected Pseudomonas strains for biocontrol of Rhizoctonia solani in potato. Phytopathologia Mediterranea, 52: 449-456.

Narisawa, K., K. Ohki and T. Hashiba. 2000. Suppression of clubroot and Verticillium yellows in Chinese cabbage in the field by the root endophytic fungus, Heteroconium chaetospira. Plant Pathology, 49: 141-146.

Nefzi, A., R. Aydi Ben Abdallah, H. Jabnoun-Khiareddine, N. Ammar, L. Somai and M. Daami-Remadi. 2018. Biostimulation of tomato growth and suppression of Fusarium crown and root rot disease using fungi naturally associated to Lycium arabicum. Journal of Agricultural Science and Food Research, 9: 200-216.

Olson, H. A. and D. M. Benson. 2007. Induced systemic resistance and the role of binucleate Rhizoctonia and Trichoderma hamatum 382 in biocontrol of Botrytis blight in geranium. Biological Control, 42: 233-241.

Palanichamy, P., G. Krishnamoorthy, S. Kannan and M. Marudhamuthu. 2018. Bioactive potential of secondary metabolites derived from medicinal plant endophytes. Egyptian Journal of Basic and Applied Sciences, 5: 303-312.

Parnell, J. J., R. Berka, H. A. Young, J. M. Sturino, Y. Kang, D. Barnhart and M. V. DiLeo. 2016. From the lab to the farm: An industrial perspective of plant beneficial microorganisms. Frontiers in Plant Science, 7: 1110.

Schulz, B., C. Boyle, S. Draeger, A. Römmert and K. Krohn. 2002. Endophytic fungi: A source of novel biologically active secondary metabolites. Mycological Research, 106: 996-1004.

Shakoor, S., M. Inam-ul-Haq, S. Bibi and R. Ahmed. 2015. Influence of root inoculations with vasicular arbuscular mycorrhizae and rhizomyx for the management of root rot of chickpea. Pakistan Journal of Phytopathology, 27: 153-158.

Sreevidya, M., S. Gopalakrishnan, T. M. Melø, N. Simic, P. Bruheim, M. Sharma, V. Srinivas and G. Alekhya. 2015. Biological control of Botrytis cinerea and plant growth promotion potential by Penicillium citrinum in chickpea (Cicer arietinum L.). Biocontrol Science and Technology, 25: 739-755.

Strobel, G. 2006. Muscodor albus and its biological promise. Journal of Industrial Microbiology and Biotechnology, 33: 514-522.

Strobel, G. A. 2003. Endophytes as sources of bioactive products. Microbes and Infection, 5: 535-544.

Tan, R. X. and W. X. Zou. 2001. Endophytes: A rich source of functional metabolites. Natural Product Reports, 18: 448-459.

Tarhouni, B. 2007. Chemical control of Rhizoctonia solani. Annual Report of the Technical Center of Potato, Essaida, Tunisia, pp. 81e86.

Vibha, S. 2010. Effect of fungal metabolites and amendments on mycelial growth of Rhizoctonia solani. Journal of Plant Protection Research, 50: 93-97.

Wang, Q., E. Zhang, F. Li and F. Li. 2008. Runoff efficiency and the technique of micro-water harvesting with ridges and furrows, for potato production in semi-arid areas. Water Resources Management, 22: 1431-1443.

Wang, W., Y. Zhai, L. Cao, H. Tan and R. Zhang. 2016. Endophytic bacterial and fungal microbiota in sprouts, roots and stems of rice (Oryza sativa L.). Microbiological Research, 188: 1-8.

Wood, E. M., T. D. Miles and P. S. Wharton. 2013. The use of natural plant volatile compounds for the control of the potato postharvest diseases, black dot, silver scurf and soft rot. Biological Control, 64: 152-159.

Full Text: PDF

DOI: 10.33687/phytopath.011.01.3897


  • There are currently no refbacks.

Copyright (c) 2022 Rania Aydi-Ben Abdallah, Hayfa Jabnoun-Khiareddine, Fakher Ayed, Mejda Daami-Remadi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.