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Complete Nucleotide Sequence Analysis of RNA2 and Phylogenetic Analyses of a Paprika Isolate of Broad Bean Wilt Virus in Korea
Abstract
An isolate of Broad bean wilt virus 2 (BBWV 2), which was isolated from paprika showed spotty fruits. Host range of BBWV 2 was nine species including Chenopodium amaranticolor, and could be characterized by the symptoms include chlorotic local and distortion on upper leaves of C. amaranticolor. Datura stramonium induced chlorotic spot on uninoculated upper leaves. The purified virus particles were isometric about 25 nm in diameter. The genomic RNA of this virus consisted of two components of about 6.3 kb and 4.5 kb molecules and coat protein contained two polypeptide species which calculated with about 42 kDa and 22 kDa. We determined the complete sequence of RNA2 from Broad bean wilt virus 2 isolate Pa. The complete nucleotide (nt) sequence of RNA 2 for BBWV 2-Pa was 3,597 nt in length. encoding a pupative movement protein (MP) and coat protein (CP). Between BBWV 2-Pa and other serotype II favaviruses, the overall amino acid similarities in the polyprotein coding regions was and 84%-93% for RNA2. In the 5′UTR, the nucleotide similarity was 65%-92%. For the 3′UTRs, they are 38%-60%, respectively. For the Genome organization of BBWV 2-Pa was similar to other comoviruses in the Comoviridae.
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References
Boccardo, G. and M. Conti. 1973. Purification and properties of nasturium ringsopt virus. Phytopath Z 78:14-34.
Choi, H.S., J.D. Cho, K.H. Lee and J.S. Kim. 2001. Broad bean wilt fabavirus and their specific ultrastructures. Korean J. Electron Microscopy. 31:215-222.
Conti, M., V. Lisa and G. Boccardo. 1972. Preliminary results on pepper virus disease in piemonte and umbria. Ann. Fac. Sci. Agr. Unv. Torino. 7:290-301.
Cooper, J.I. 1994. Fabaviruses. In: Webster R.G. and Granoff (eds) Encyclopedia of virology. America Press, New York, pp 451-454.
Goldbach, R., G.P. Maartelli and R.G. Milne. 1995. Family Comoviridae. In: Murphy, F.A., Fauquest, C.M., Bishop, D.H.L., Ghabrial, S.A., Jarvis, A.W., Martell, G.P., Mayo, M.A., Summers, M.D. (eds) Virus taxonomy. Classification and nomenclature of viruses: sixth report of the International Committee on Taxonomy of Viruses. Springer-Verlag, Berlin Heidelberg Wein New York, pp 341-347.
Holness, C.L., G.P. Lonomossoff, D. Evans and A.J. Maule. 1989. Identification of the initiation codons for translation of cowpea mosaic virus middle component RNA using site-directed mutagenesis of an infectious cDNA colne. Virology. 172:311-320.
Hong, J.S. 1998. Properties of an isolate of broad been wilt vrirus from pepper. In M.A. Thesis of Kangwon National University, Korea.
Ikegami, M., H. Kawashima, T. Natsuaki and N. Sugimura. 1998. Complete nucleotide sequence of the genome organization of RNA 2 of patchouli mild mosaic virus, a new fabavirus. Arch Virol. 143:2431-2434.
Koh, L.H., J.I. Cooper and S.M. Wong. 2001. Complete sequence and phylogenetic analyses of a Singapore isolate of a broad beam wilt fabavirus. Arch Virol. 146:135-147.
Laemmli, U.K. and M. Farre. 1973. Maturation of the head of bacteriophage T4. I. DNA packaging event. J. Mol. Biol. 80:575-599.
Lockhare, B.E.L. and H.U. Fisher. 1977. Some properties of an isolate of broad bean wilt virus associated with a field disease of pepper in Morocco. Phytopath. Z. 88:209-214.
Margis, R., M. Viry, M. Pinck, N. Bardonnet and L. Pinck. 1994. Differential proteolytic activities of precursor and mature form of the 24K protease of grape vine fanleaf nepovirus. Virology. 200:79-86.
Murphy, F.A., C.M. Fauquet, D.H.L. Bishop, S.A. Ghabrial, A.W. Jarvis, G.P. Martelli, M.A. Mayo and M.D. Summers. 1995. Virus Taxonomy. Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses, Springer, New York (Arch Virol [Suppl.] 10).
Nakamura, S., T. Iwai and R. Honkura. 1998. Complete nucleotide sequence and genome organization of broad bean wilt virus 2. Ann. Phytopathol. Soc. Jpn. 64:565-568.
Qi, Y., X. Zhou and D. Li. 2000. Complete nucleotide sequence and infectious cDNA clone of the RNA1 of a Chinese isolate of Broad bean wilt virus 2. Prog. Nat. Sci. 10:680-686.
Ryu, K.H., C.W. Choi, J.K. Choi and W.M. Park. 1995. Cloning of the 3’-terminal region encoding movement and coat proteins of a Korean isolate of odontoglossum ringspot virus. Arch. Virol. 140:481-490.
Shukla, D.D. K.H. Gough. 1983. Tobacco streak, broad bean wilt, cucumber mosaic, and alfalfa mosaic viruses associated with ring spot ajuga reptans in Australia. Plant Dis. 67:221-224.
Stubbs, L.L. 1947. Adestructive vascular wilt virus disease of broad bean (Vicia faba L.) in Victoria. J Dept Agric Victoria. 46:323-332.
Taylor, R.H. and L.L. Stubbs. 1972. Broad bean wilt virus. CMI/AAB Description of plant viruses. No. 81.
Uyemoto, J.K. and R. Provvidenti. 1974. Isolation and identification of Broad bean wilt virus. Phytopathol. 64:1547-1548.
Vega, J., O. Gracia and J.M. Feldman. 1980. Characteristics of the Argentine broad bean wilt virus. Cytopathological aspects. Phytopath. Z 99:242-250.
Xu, Z.G., A.J. Cickbain, R.D. Wood and D.A. Govier. 1988. The serological relationships and some other properties of isolates of broad bean wilt virus from faba bean and pea in China. Ann. Appl. Biol. 113:287-296.
Yankulova, M. andP. Kaitazova. 1979. Broad bean wilt virus-a new virus on pepper in Bulgaria. Gradinarska Lozarska Nanka. 16:48-57.
DOI: 10.33687/phytopath.003.01.0377
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