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Activation of Capsicum annuum L. Defense System against Fusarium Wilt through Plant Activators and their Impact on Horticultural Attributes

Muhammad Atiq, Sameen Adil, Nasir A. Rajput, Shahbaz T. Sahi, Muhammad Usman, Shahid Iqbal, Shahid A. Chand, Ahmad Nawaz, Asif M. Arif, Azeem Akram, Hamza Shahbaz

Abstract


Capsicum annuum L. member of family Solanaceae is an important vegetable crop throughout the world. Fusarium wilt of chilli caused by Fusarium oxysporoum f.sp. capsici, is a serious destructive disease, which reduces its yield and is a major threat to the economy. Plant activators play an important role to manage soil born disease. In current study, a pot experiment was carried out under greenhouse conditions in the research area of Plant Pathology Department, University of Agriculture, Faisalabad. Five plant activators K2HPO4, CaCl2, Benzoic acid, Salicylic acid and Alpha-Tocopherol were used to manage the Fusarium wilt of chilli. Soil drenching of each plant activator was applied at three different concentrations 0.25%, 0.5% and 0.75% after transplantation. Results showed that the minimum incidence of disease, maximum length of shoot, length of root was revealed by K2HPO4 at 0.75% concentration. While benzoic acid exhibit maximum shoot fresh weight, maximum shoot dry weight, fresh root weight and dry root weight at 0.75% concentration. Maximum fresh leaf weight showed by salicylic acid whereas maximum dry leaf weight exhibited by alpha-tocopherol at 0.75% concentration. It was concluded that K2HPO4 at 0.75% concentration is effective to manage Fusarium wiltof chilli that can be used to manage disease in future.

Keywords


Induced resistance; K2HPO4; CaCl2; Benzoic acid; Salicylic acid; Alpha-Tocopherol

References


Abdel-Monaim, M. F. and M. E. Ismail. 2010. The use of antioxidants to control root rot and wilt diseases of pepper. Notulae Scientia Biologicae, 2: 46-55. https://doi.org/10.15835/nsb223699

Agrios, G. N. 2005. Plant Pathology. Academic Press: San Diego, CA.

Alabi, D. 2006. Effects of fertilizer phosphorus and poultry droppings treatments on growth and nutrient components of pepper (Capsicum annuum L). African Journal of Biotechnology, 5: 671-77.

Ali, Q., M. Khan and M. Ashfaq. 2018. Efficiency analysis of off-season capsicum/bell pepper production in Punjab-Pakistan: A DEA approach. The Journal of Animal and Plant Sciences, 28: 1508-15.

Ali, Z., M. Shah, A. Nawaz, M. Shahjahan, H. Butt, M. Shahid and R. Ahmed. 2014. Assessment of induced systemic resistance through antagonistic rhizobacterial potential with salicylic acid against karnal bunt of wheat. Pakistan Journal of Phytopathology, 26: 253-58.

Ali, Z., I. Smith and D. I. Guest. 2000. Combinations of potassium phosphonate and Bion (acibenzolar-S-methyl) reduce root infection and dieback of Pinus radiata, Banksia integrifolia and Isopogon cuneatus caused by Phytophthora cinnamomi. Australasian Plant Pathology, 29: 59-63.

Ashfaq, M., S. Iqbal, T. Mukhtar and H. Shah. 2014. Screening for resistance to cucumber mosaic cucumovirus in chilli pepper. Journal of Animal and Plant Sciences, 24: 791-95.

Baenas, N., M. Belović, N. Ilic, D. Moreno and C. García-Viguera. 2019. Industrial use of pepper (Capsicum annum L.) derived products: Technological benefits and biological advantages. Food chemistry, 274: 872-85. https://doi.org/10.1016/j.foodchem.2018.09.047; PMid:30373022

Bashir, N., M. Hashmi and F. Jamil. 1997. Induction of systemic acquired resistance by oxalic acid in chickpea (Cicer arientinum L.) against Ascochyta rabiei. Pakistan Journal of Phytopathology, 9: 18-20.

Basu, S. K. and A. K. De. 2003. Capsicum: Historical and botanical perspectives. In, Capsicum. CRC Press.

Biswas, S., N. Pandey and M. Rajik. 2012. Inductions of defense response in tomato against Fusarium wilt through inorganic chemicals as inducers. Journal of Plant Pathology and Microbiology, 4: 128-35.

Chakraborty, S. and I. Pangga. 2004. Plant disease and climate change. In, Plant Microbiology. Taylor and Francis. London UK.

Chet, I., Y. Elad, A. Kalfon, Y. Hadar and J. Katan. 1982. Integrated control of soilborne and bulbborne pathogens in iris. Phytoparasitica, 10: 229-35. https://doi.org/10.1007/BF03023963

Chong, T., M. Abdullah, N. Fadzillah, O. Lai and N. Lajis. 2005. Jasmonic acid elicitation of anthraquinones with some associated enzymic and non-enzymic antioxidant responses in Morinda elliptica. Enzyme and Microbial Technology, 36: 469-77. https://doi.org/10.1016/j.enzmictec.2004.11.002

Colson-Hanks, E., S. Allen and B. Deverall. 2000. Effect of 2, 6-dichloroisonicotinic acid or benzothiadiazole on Alternaria leaf spot, bacterial blight and Verticillium wilt in cotton under field conditions. Australasian Plant Pathology, 29: 170-77. https://doi.org/10.1071/AP00030

Costa, T. R., O. F. Fernandes, S. C. Santos, C. l. M. Oliveira, L. M. Lião, P. H. Ferri, J. R. Paula, H. D. Ferreira, B. H. Sales and R. S. Maria do Rosário. 2000. Antifungal activity of volatile constituents of Eugenia dysenterica leaf oil. Journal of ethnopharmacology, 72: 111-17. https://doi.org/10.1016/S0378-8741(00)00214-2

Dekker, J. 1976. Acquired resistance to fungicides. Annual Review of Phytopathology, 14: 405-28. https://doi.org/10.1146/annurev.py.14.090176.002201

Devika Rani, G., M. Naik, K. Raju and P. Prasad. 2007. Prevalence of wilt of chilli and assessment of population dynamics of Fusarium in predominant chilli (Capsicum annuum L.) growing regions of Karnataka. Journal of Soil Biology and Ecology, 27: 50-61.

Dubey, S. C., M. Suresh and B. Singh. 2007. Evaluation of Trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological control, 40: 118-27. https://doi.org/10.1016/j.biocontrol.2006.06.006

Duru, M., S. Kordali, A. Cakir, H. Zengin and M. E. Duru. 2003. Antifungal activities of the leaves of three Pistacia species grown in Turkey. Fitoterapia, 74: 164-67. https://doi.org/10.1016/S0367-326X(02)00320-9

Ekundayo, E., F. Adetuyi and F. Ekundayo. 2011. In vitro antifungal activities of bacteria associated with maize husks and cobs. Research journal of microbiology, 6: 418-23. https://doi.org/10.3923/jm.2011.418.424

El-Yazeid, A. 2011. Effect of foliar application of salicylic acid and chelated zinc on growth and productivity of sweet pepper (Capsicum annuum L.) under autumn planting. Research journal of agriculture and biological sciences, 7: 423-33.

FAOSTAT. 2016. Food and agriculture organization of the United Nations. FAOSTAT Statistics Database.

FAOSTAT. 2017. Food and agriculture organization of the United Nations. FAOSTAT Statistics Database.

Gantait, A., A. Maji, T. Barman, P. Banerji, P. Venkatesh and P. K. Mukherjee. 2012. Estimation of capsaicin through scanning densitometry and evaluation of different varieties of capsicum in India. Natural product research, 26: 216-22. https://doi.org/10.1080/14786419.2010.535169; PMid:21834628

Ghini, R., E. Hamada and W. Bettiol. 2008. Climate change and plant diseases. Scientia Agricola, 65: 98-107. https://doi.org/10.1590/S0103-90162008000700015

Govindappa, M., V. R. Rai and S. Lokesh. 2011. In vitro and In vivo responses of different treating agents against wilt disease of safflower. Journal of Cereals and Oilseeds, 2: 16-25.

Hanieh, A., D. Mojtaba, Z. Zabihollah and A. Vahid. 2013. Effect of pre-sowing salicylic acid seed treatment on seed germination and growth of greenhouse sweet pepper plants. Indian Journal of Science and Technology, 6: 3868-71. https://doi.org/10.17485/ijst/2013/v6i1.13

Hayat, S., B. Ali and A. Ahmad. 2007. Salicylic acid: Biosynthesis, metabolism and physiological role in plants. In, Salicylic Acid: A Plant Hormone. Springer. https://doi.org/10.1007/1-4020-5184-0

Hayat, S., Q. Fariduddin, B. Ali and A. Ahmad. 2005. Effect of salicylic acid on growth and enzyme activities of wheat seedlings. Acta Agronomica Hungarica, 53: 433-37. https://doi.org/10.1556/AAgr.53.2005.4.9

Howard, L., S. Talcott, C. Brenes and B. Villalon. 2000. Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum species) as influenced by maturity. Journal of agricultural and food chemistry, 48: 1713-20. https://doi.org/10.1021/jf990916t; PMid:10820084

Huang, J. S. and H. T. Hsu. 2003. Induced resistance in plant. In: H. C Huang and S. N. Acharya (eds.), Advances in Plant Disease Management. Research. Sign Post Trivan.

Huang, X.-F., J.-Y. Xue, A.-Q. Jiang and H.-L. Zhu. 2013. Capsaicin and its analogues: Structure-activity relationship study. Current Medicinal Chemistry, 20: 2661-72. https://doi.org/10.2174/0929867311320210004; PMid:23627937

Huynh, Q. K., J. R. Borgmeyer, C. E. Smith, L. D. Bell and D. M. Shah. 1996. Isolation and characterization of a 30 kDa protein with antifungal activity from leaves of Engelmannia pinnatifida. Biochemical Journal, 316: 723-27. https://doi.org/10.1042/bj3160723; PMid:8670144 PMCid:PMC1217410

Hyder, S., A. S. Gondal, Z. F. Rizvi, R. Ahmad, M. M. Alam, A. Hannan, W. Ahmed, N. Fatima and M. Inam-ul-Haq. 2020. Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Scientific Reports, 10: 1-15. https://doi.org/10.1038/s41598-020-69410-3; PMid:32807801 PMCid:PMC7431856

Inbar, M., H. Doostdar, R. M. Sonoda, G. L. Leibee and R. T. Mayer. 1998. Elicitors of plant defensive systems reduce insect densities and disease incidence. Journal of Chemical Ecology, 24: 135-49. https://doi.org/10.1023/A:1022397130895

Jalali, B., S. Bhargava and A. Kamble. 2006. Signal transduction and transcriptional regulation of plant defence responses. Journal of Phytopathology, 154: 65-74. https://doi.org/10.1111/j.1439-0434.2006.01073.x

Khan, W., B. Prithiviraj and D. L. Smith. 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of plant physiology, 160: 485-92. https://doi.org/10.1078/0176-1617-00865; PMid:12806776

Larkin, R. P. and D. R. Fravel. 2002. Effects of varying environmental conditions on biological control of Fusarium wilt of tomato by nonpathogenic Fusarium spp. Phytopathology, 92: 1160-66. https://doi.org/10.1094/PHYTO.2002.92.11.1160; PMid:18944240

Lee, S.-O., G.-J. Choi, K.-S. Jang, H.-K. Lim, K.-Y. Cho and J.-C. Kim. 2007. Antifungal activity of five plant essential oils as fumigant against postharvest and soilborne plant pathogenic fungi. The Plant Pathology Journal, 23: 97-102. https://doi.org/10.5423/PPJ.2007.23.2.097

Limón, M. C., R. Rodríguez-Ortiz and J. Avalos. 2010. Bikaverin production and applications. Applied microbiology and biotechnology, 87: 21-29. https://doi.org/10.1007/s00253-010-2551-1; PMid:20376635

Madbouly, A. K. and A. Abdelbacki. 2017. Biocontrol of certain soilborne diseases and promotion of growth of Capsicum annuum using biofungicides. Pakistan Journal of Botany, 49: 371-78.

Mahmood, R. and U. Bashir. 2011. Relationship between soil physicochemical characteristics and soil-borne diseases. Mycopath, 9: 87-93.

Mann, A. 2011. Biopotency role of culinary spices and herbs and their chemical constituents in health and commonly used spices in Nigerian dishes and snacks. African Journal of Food Science, 5: 111-24.

Maruti, T., B. Tembhurne, R. Chavan and Y. Amaresh. 2014. Reaction of chilli (Capsicum annuum L.) genotypes and hybrids against Fusarium wilt (Fusarium solani). Journal of Spices and Aromatic Crops, 23: 186-91.

Matthew, A., A. Lawal, P. Chindo and O. Banwo. 2006. Outbreak of basal stem rot and wilt disease of pepper in northern Nigeria. Journal of Plant Protection Research, 46: 7-14.

Mina, U. and S. Dubey. 2010. Effect of environmental variables on development of Fusarium wilt in chickpea (Cicer arietinum) cultivars. Indian Journal of Agricultural Sciences, 80: 231-34.

MOF. 2015-2016. Economic Survey of Pakistan. Ministry of Finance, Government of Pakistan. Islamabad, Pakistan.

Naik, M., H. Madhukar and G. Devika Rani. 2007. Evaluation of fungicides against Fusarium solani, the causal agent of wilt of chilli. Vegetable Science, 34: 173-76.

Naik, M., G. D. Rani and H. Madhukar. 2008. Identification of resistant sources against wilt of chilli (Capsicum annuum L.) caused by Fusarium solani (Mart.) Sacc. Journal of Mycopathological Research, 46: 93-96.

Nawaz, K., A. Shahid, M. Subhani, W. Anwar and M. Aslam. 2016. First report of Pythium spinosum causing root rot of chili (Capsicum annuum) in Pakistan. Plant Disease, 100: 526-26. https://doi.org/10.1094/PDIS-07-15-0745-PDN

Nawaz, M. A., F. Shireen, Y. Huang, Z. Bie, A. Waqar and B. A. Saleem. 2017. Perspectives of vegetable grafting in Pakistan: Current status, challenges and opportunities. International Journal of Agriculture and Biology, 19: 1165-74. https://doi.org/10.17957/IJAB/15.0404

Niehaus, E.-M., K. W. von Bargen, J. J. Espino, A. Pfannmüller, H.-U. Humpf and B. Tudzynski. 2014. Characterization of the fusaric acid gene cluster in Fusarium fujikuroi. Applied microbiology and biotechnology, 98: 1749-62. https://doi.org/10.1007/s00253-013-5453-1; PMid:24389666

Pavlovkin, J., I. Mistrik and M. Prokop. 2004. Some aspects of the phytotoxic action of fusaric acid on primary Ricinus roots. Plant Soil and Environment, 50: 397-401. https://doi.org/10.17221/4050-PSE

Quresh, W., M. Alam, H. Ullah, S. A. Jatoi and W. U. Khan. 2015. Evaluation and characterization of Chilli (Capsicum annuum L.) germplasm for some morphological and yield characters. Pure and Applied Biology, 4: 628-36. https://doi.org/10.19045/bspab.2015.44023

Ram, D., K. Mathur, B. C. Lodha and J. Webster. 2000. Evaluation of resident biocontrol agents as seed treatments against ginger rhizome rot. Indian Phytopathology, 53: 450-54.

Rekah, Y., D. Shtienberg and J. Katan. 2001. Population dynamics of Fusarium oxysporum f. sp. radicis-lycopersici in relation to the onset of Fusarium crown and root rot of tomato. European Journal of Plant Pathology, 107: 367-75.

Russo, V. M. and L. R. Howard. 2002. Carotenoids in pungent and non‐pungent peppers at various developmental stages grown in the field and glasshouse. Journal of the Science of Food and Agriculture, 82: 615-24. https://doi.org/10.1002/jsfa.1099

Saikia, R., S. Varghese, B. P. Singh and D. K. Arora. 2009. Influence of mineral amendment on disease suppressive activity of Pseudomonas fluorescens to Fusarium wilt of chickpea. Microbiological research, 164: 365-73. https://doi.org/10.1016/j.micres.2007.05.001; PMid:17604612

Saremi, H. and M. E. Amiri. 2010. Exploration of potato cultivar resistant to the major fungal pathogen on potato wilting disease in Iran. Journal of Food, Agriculture and Environment, 8: 821-26.

Saremi, H., L. Burgess and D. Backhouse. 1999. Temperature effects on the relative abundance of Fusarium species in a model plant-soil ecosystem. Soil Biology and Biochemistry, 31: 941-47. https://doi.org/10.1016/S0038-0717(99)00001-2

Sarwar, N., C. Zahid, I. Haq and F. Jamil. 2005. Induction of systemic resistance in chickpea against Fusarium wilt by seed treatment with salicylic acid and Bion. Pakistan Journal of Botany, 37: 989-96.

Saxena, A., R. Sahni, H. Yadav, S. Upadhyay and M. Saxena. 2005. Antifungal activity of some higher plants against Fusarium oxysporum f. sp. pisi. Living World journal, 12: 32-39.

Sharma, R., B. Singh, M. Thakur and K. Verma. 2002. Chemical management of linseed wilt caused by Fusarium oxysporum f. sp. lini. Annals of Plant Protection Sciences, 10: 390-91.

Singh, G., S. Maurya, C. Catalan and M. De Lampasona. 2004. Chemical constituents, antifungal and antioxidative effects of ajwain essential oil and its acetone extract. Journal of agricultural and food chemistry, 52: 3292-96. https://doi.org/10.1021/jf035211c; PMid:15161185

Sitara, U. and N. Hasan. 2011. Studies on the efficacy of chemical and non chemical treatments to control mycoflora associated with chilli seed. Pakistan Journal of Botany, 43: 95-110.

Sitara, U., I. Niaz, J. Naseem and N. Sultana. 2008. Antifungal effect of essential oils on in vitro growth of pathogenic fungi. Pakistan Journal of Botany, 40: 409-14.

Sridhar, S. R., R. V. Rajagopal, R. Rajavel, S. Masilamani and S. Narasimhan. 2003. Antifungal activity of some essential oils. Journal of agricultural and food chemistry, 51: 7596-99. https://doi.org/10.1021/jf0344082; PMid:14664513

Stewart, W., D. Dibb, A. Johnston and T. Smyth. 2005. The contribution of commercial fertilizer nutrients to food production. Agronomy journal, 97: 1-6. https://doi.org/10.2134/agronj2005.0001

Suharja and Sutarno. 2009. Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments. Nusantara Bioscience, 1: 6-16.

Tesfaw, A. 2013. Benefit-cost analysis of growing pepper: A trial at west Gojjam, near the source of blue Nile. International Journal of Agriculture and Crop Sciences, 6: 1203-07.

Torres-Castillo, J., S. Sinagawa-García, G. Martínez-Ávila, A. López-Flores, E. Sánchez-González, V. Aguirre-Arzola, R. Torres-Acosta, E. Olivares-Sáenz, E. Osorio-Hernández and A. Gutiérrez-Díez. 2013. Moringa oleifera: Phytochemical detection, antioxidants, enzymes and antifugal properties. International Journal of Experimental Botany, 82: 193-202.

Vanitha, S., S. Niranjana, C. Mortensen and S. Umesha. 2009. Bacterial wilt of tomato in Karnataka and its management by Pseudomonas fluorescens. Biocontrol, 54: 685-95. https://doi.org/10.1007/s10526-009-9217-x

Vega-Gálvez, A., K. Di Scala, K. Rodríguez, R. Lemus-Mondaca, M. Miranda, J. López and M. Perez-Won. 2009. Effect of air-drying temperature on physico-chemical properties, antioxidant capacity, colour and total phenolic content of red pepper (Capsicum annuum, L. var. Hungarian). Food chemistry, 117: 647-53. https://doi.org/10.1016/j.foodchem.2009.04.066

Wiemann, P., C. M. Sieber, K. W. Von Bargen, L. Studt, E.-M. Niehaus, J. J. Espino, K. Huss, C. B. Michielse, S. Albermann and D. Wagner. 2013. Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites. PLoS pathogens, 9: e1003475. https://doi.org/10.1371/journal.ppat.1003475; PMid:23825955 PMCid:PMC3694855

Wu, H.-s., Y. Wang, W. Bao, D.-y. Liu, W. Raza, Q.-w. Huang, Z.-s. Mao and Q.-r. Shen. 2009. Responses of Fusarium oxysporum f. sp. niveum to exogenously added sinapic acid in vitro. Biology and fertility of soils, 45: 443-47. https://doi.org/10.1007/s00374-009-0353-3

Ye, X. and T. Ng. 2002. A new antifungal peptide from rice beans. The Journal of peptide research, 60: 81-87. https://doi.org/10.1034/j.1399-3011.2002.20962.x; PMid:12102720

Yousaf, A., J. Wu, Q. Shakeel, Y. Iftikhar, M. I. Ullah, U. Tahira, M. Mubeen and W. Dong. 2017. Evaluation of resistance of Capsicum annuum against Meloidogyne incognita and Sclerotium rolfsii and their integrated management. Pakistan Journal of Zoology, 49: 117-23. https://doi.org/10.17582/journal.pjz/2017.49.5.1671.1682


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DOI: 10.33687/phytopath.010.03.3623

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