Impact of Farm Management Practices on Downy Mildew Disease of Cucumber in High Tunnels

Muhammad N. Shahid, Muhammad Abid, Sobia Chohan, Muhammad Binyameen, Muhammad Sajid


Downy mildew of cucumber caused by Pseudoperonospora cubensisis is a serious threat to the cucurbits production in high tunnels. In this study, the disease incidence and severity were recorded from nine high tunnel farms, located at Vehari District, Pakistan. The results revealed that downy mildew incidence was ranged from 24 to 80%. The disease development was correlated with plant genotype, sowing time, commonly used fungicides, and plant growth stages. "Kaan" was the most resistant while "Yayla" was the highly susceptible plant genotype against the disease. Cucumber sowing at mid-November showed significantly less disease over early and late sowing. Flowering was the most vulnerable stage of plants for disease development. ‘Champion’ (Copper hydroxide) followed by ‘Score’ (Difenoconazole) fungicides reduced the disease to 55% and 43%, respectively, which increased the yield 8-15 %. We conclude that the use of resistant genotype “Kaan”, protectant fungicide ‘Champion’ and appropriate sowing time Mid-November may reduce the disease incidence appreciably in high tunnels.


Downy mildew; Pseudoperonospora cubensis; Cucumber; plant resistance; sowing time; fungicide; high tunnels


Ali Q, Yaseen MR and Khan MTI. 2019. Energy budgeting and greenhouse gas emission in cucumber under tunnel farming in Punjab, Pakistan. Scientia Horticulturae; 250: 168-173.

Ando, K., K.M. Carr and R. Grumet. 2012. Transcriptome analyses of early cucumber fruit growth identifies distinct gene modules associated with phases of development. Bio Med Central genomics, 13: 518.

Choi, Y.J., S.B. Hong and H.D. Shin. 2005. A re-consideration of Pseudoperonospora cubensis and P. humuli based on molecular and morphological data. Mycological Research, 109: 841-848.

Cohen, Y. 1977. The combined effects of temperature, leaf wetness, and inoculum concentration on infection of cucumbers with Pseudoperonospora cubensis. Canadian Journal Botany, 55: 1478-1487.

Cohen, Y., A. Rubin and M. Galperin. 2016. Formation and infectivity of oospores of Pseudoperonospora cubensis, the causal agent of downy mildew in cucurbits. Horticultural Reviews, 279: 314.

Colucci, S. J. 2008. Host range, fungicide resistance and management of Pseudoperonospora cubensis, causal agent of cucurbit downy mildew. North Carolina State University, Raleigh, North Carolina, Master of Science.

Delmas, C.E., Y. Dussert, L. Delière, C. Couture, I. D. Mazet, S. Richart Cervera and F. Delmotte. 2017. Soft selective sweeps in fungicide resistance adaptation: recurrent mutations without fitness costs in grapevine downy mildew. Molecular Ecology, 26: 1936-1951.

Ding, X., Y. Jiang, T. Hao, H. Jin, H. Zhang, L. He, Q. Zhou, D. Huang, D. Hui and J. Yu. 2016. Effects of heat shock on photosynthetic properties, antioxidant enzyme activity, and downy mildew of cucumber (Cucumis sativus L.). Public Library of Science One, 11: e0152429.

Dodds, P.N. and J.P. Rathjen. 2010. Plant immunity: towards an integrated view of plant-pathogen interactions. Nature Reviews Genetics, 11: 539-548.

Garrett, K.A., S.P. Dendy, E.E. Frank, M.N. Rouse and S.E. Travers. 2006. Climate change effects on plant disease: genomes to ecosystems. Annual Review of Phytopathology, 44: 489-509.

Ghini, R., E. Hamada, R.R. Gonçalves, L. Gasparotto and J.C.R. Pereira. 2007. Análise de risco das mudanças climáticas globais sobre a sigatoka-negra da bananeira no Brasil. Fitopatologia Brasileira, 32: 197-204.

Gupta S. and M. Gupta 2018. Diseases of vegetables under protected cultivation conditions. Plant Disease Research, 33: 1-14.

Hijmans, R., G. Forbes and T. Walker. 2000. Estimating the global severity of potato late blight with GIS‐linked disease forecast models. Plant Pathology, 49: 697-705.

Juroszek, P. and A. Von Tiedemann. 2011. Potential strategies and future requirements for plant disease management under a changing climate. Plant Pathology, 60: 100-112.

Ketta, H., S.Kamel, A. Ismail and E. Ibrahem. 2016. Control of Downy Mildew Disease of Cucumber Using Bacillus chitinosporus. Egyptian Journal of Biological Pest Control, 26: 839-845.

Lebeda, A. and M.P. Widrlechner. 2003. A set of Cucurbitaceae taxa for differentiation of Pseudoperonospora cubensis pathotypes/Ein Testsortiment von Cucurbitaceae-Taxa für die Differenzierung der Pathotypen von Pseudoperonospora cubensis. Journal of Plant Diseases and Protection, 337-349.

Lebeda, A. and T. Jendrulek. 1988. Application of methods of multivariate analysis in comparative epidemiology and research into field resistance/Anwendung der multivariaten Analyse in der vergleichenden Epidemiologie und der Erforschung der Feldresistenz. Journal of Plant Diseases and Protection, 95 (5): 495-505.

Lebeda, A. and Y. Cohen. 2011. Cucurbit downy mildew (Pseudoperonospora cubensis) biology, ecology, epidemiology, host-pathogen interaction and control. European Journal of Plant Pathology, 129: 157-192.

Legrève, A. and E. Duveiller. 2010. Preventing potential diseases and pest epidemics under a changing climate. Climate Change and Crop Production, 1: 50-70.

Palti, J. and Y. Cohen. 1980. Downy mildew of cucurbits (Pseudoperonospora cubensis): the fungus and its hosts, distribution, epidemiology and control. Phytoparasitica, 8: 109-147.

Savory, E.A., L.L. Granke, L.M. Quesada-Ocampo, M. Varbanova, M.K. Hausbeck and B. Day. 2011. The cucurbit downy mildew pathogen Pseudoperonospora cubensis. Molecular Plant Pathaology, 12: 217-226.

Shetty, N.V., T.C. Wehner, C.E. Thomas, R.W. Doruchowski and K.V. Shetty. 2002. Evidence for downy mildew races in cucumber tested in Asia, Europe, and North America. Scientia Horticulturae, 94: 231-239.

Thomas, A., 2016. Biology, Epidemiology and Population Genomics of Pseudoperonospora cubensis, the Causal Agent of Cucurbit Downy Mildew North Carolina State University.

Urban, J. and A. Lebeda. 2006. Fungicide resistance in cucurbit downy mildew-methodological, biological and population aspects. Annals of Applied Biology, 149: 63-75.

Vanden Langenberg, K.M. and T.C. Wehner. 2016. Downy Mildew Disease Progress in Resistant and Susceptible Cucumbers Tested in the Field at Different Growth Stages. HortScience, 51: 984-988.

Wolfe, D.W., L. Ziska, C. Petzoldt, A. Seaman, L. Chase and K. Hayhoe. 2008. Projected change in climate thresholds in the Northeastern US: implications for crops, pests, livestock, and farmers. Mitigation and Adaptation Strategies for Global Change, 13: 555-575.

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DOI: 10.33687/phytopath.009.03.3457


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