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Detection and Identification of Mycobiota Associated with Rice in Three Districts of Iraq
Abstract
The reduction of yield, quality, and nutritional value of grain cereals by different fungal species is of great concern around the world. A comprehensive investigation was made on fungal mycobiota in rice from three provinces; Baghdad, Al-Najaf and Al-Qadisiyah of Iraq surveyed during the growing season 2014-2015. Fields were selected due to their location near the Euphrates and Tigris Rivers. Mycological analysis indicated that there were a difference rates in disease infection due to various fungal species. Predominant fungal species identified as Bipolaris spicifera, Curvularia lunata, Fusarium spp., Nigrospora oryzae, Exserohilum rostratum, Alternaria spp. and Thanatephorus cucumeris. Confirmation of the associated fungal species was further accomplished by analysis of their ribosomal DNA and internal transcribed spacer region sequences. The present study provides a detailed description of the associated fungal species, which may help in understanding the population dynamics and in developing effective control measures.
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References
Abass, M. H., and N. H. Mohammed. 2014. Morphological, molecular and pathological study on Nigrospora oryzae and Nigrospora sphaerica, the leaf spot fungi of date palm. Basra Journal for Date Palm Researches. 13:1-2.
Abd-Elsalam, K. A., I. N. Aly, M. A. Abdel-Satar, M. S. Khalil and J. A. Verreet. 2004. PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African Journal of Biotechnology. 2(4): 82-85.
Abeysingne, S. 2007. Biological control of Fusarium solani f. sp. phaseoli the causal agent of root rot of bean using Bacillus subtilis CA32 and Trichoderma harzianum RU01. Ruhuna Journal of Science. 2:82–88.
Agrawal, T. and A. Kotasthane. 2009. A simple medium for screening chitinase activity of Trichoderma spp. methods of molecular identifications and lab protocols. 1 -7.
Ahmadpour, A., M. Donyadoost-Chelan, Z. Heidarian and M. Javan-Nikkhah. 2011. New species of Bipolaris and Curvularia on grass species in Iran. Rostaniha. 12(1): 39–49.
Agrios, G. N. 2005. Plant Pathology. Amsterdam: Elsevier Academic Press.
Al-Jubouri, A. N. H. 2010. Effect of Trichoderma harizanum Rafai on the plantation and growth of some rice varieties. J. Sci. of Kufa Uni. 2: 1.
Aye, S. S. and M. Matsumoto. 2010. Genetic Characterization by Rep-PCR of Myanmar Isolates of Rhizoctonia spp., Causal Agents of Rice Sheath Diseases. Journal of Plant Pathology.92:255-260.
Barry, M. P. and L. G. N. Robert. 2000. Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. Mycol. Res. 104 (11): 1312–1321.
Brecht, M. O., C. M. Stiles and L. E. Datnoff. 2007. Evaluation of pathogenicity Bipolaris and Curvularia spp. on dwarf and ultradwarf bermudagrasses in Florida. Online. Plant Health Progress doi:10.1094/PHP-2007-0119-02-RS.
Caracuel-Rios, Z. and J. N. Talbot. 2007. Cellular differentiation and host invasion by the rice blast fungus Magnaporthe grisea. Current Opinion in Microbiology. 10:339–345.
Chakraborty, B. N., U. Chakraborty., A. Saha., P.L. Dey. and K. Sunar. 2010. Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their 192 PCR-RAPD Profiles. Global Journal of Biotechnology and Biochemistry. 5(1): 55-61.
Desjardins, A. E. 2006. Fusarium mycotoxins : chemistry, genetics and biology.The American Phytopathological Society. St. Paul. Minnesota. APS Press. pp.184- 185.
Gao, J. X., J. Jing, Yu. C. Jin and J. Chen. 2015. Construction of a High-Quality Yeast Two-Hybrid Library and Its Application in Identifiation of Interacting Proteins with Brn1 in Curvularia lunata. Plant Pathol. J. 31(2): 108-114.
Goswami, R. S. and H. C. Kistler. 2004. Heading for disaster: Fusarium graminearum on cereal crops. Molecular Plant Pathology. 5(6): 515–525
Iram, S., and I. Ahmad. 2005. Analysis of variation in Alternaria alternata by pathogenicity and RAPD study. Polish Journal of Microbiology. 54: 13.
Izham, M. N. 2008. Morphological characteristics, distribution, and mycotoxin profiles of Fusarium species from soils in Peninsular Malaysia. MSc thesis. Universiti Sains Malaysia. Malaysia (Unpublished).
Summerell, B. A., and J. F. Leslie. 2011. Fifty years of Fusarium: how could nine species have ever been enough? Fungal Diversity. 50: 135–144.
Jacobson, E. S. 2000. Pathogenic roles for fungal melanins. Clinical Microbiology Reviews. 13: 708-717.
Jens Laurids, S., A. Elina, T. Ulf, G. Henriette and S. Teis Esben. 2013. Production of fusarielins by Fusarium. International Journal of Food Microbiology. 160: 206–211.
Jima, T. A. 2012. PCR-based identification of Fusarium spp. and impact of wound healing time on dry rot infection. Swedish University of Agricultural. Master Thesis in Plant Biology. Sweden.
Justesen, A. F., D. Yohalem, A. Bay and M. Nicolaisen. 2003. Genetic diversity in potato field populations of Thanatephorus cucumeris AG-3, revealed by ITS polymorphism and RAPD markers. Mycol Res. 107:1323–1331.
Kamaluddeen, S. S. and A. L. Abhilasha. 2013. A New Blight Disease of Rice Caused by Curvularia lunata from Uttar Pradesh. International Journal of Agricultural Science and Research. 3:13-16.
Karthikeyan, V. and S. S. Gnanamanickam. 2008. Biological control of Setaria blast (Magnaporthe grisea) with bacterial strains. Crop Protection. 27: 263–267.
Khan, A. A. and A. P. Sinha. 2005. Influence of soil and nutritional factors on the affectivity of Trichoderma harzianum against sheath blight of rice. Indian Phytopathology. 58: 276-281.
Khosravi, V., S. Naeimi, F. Padasht-Dehkaei, M. Rostami and P. C. Ceresini. 2011. First report of naturally occurring Thanatephorus cucumeris (anamorph: Rhizoctonia solani AG-1 IA) on paddy-rice fields from Iran. Iranian Journal of Plant Pathology. 47(1): 103-104.
Khush, G. S. 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Molecular Biology. 59: 1–6. (http://www.fao.org/docrep/009/a0750e/ a0750e00.HTM).
Knogge, W. 1996. Fungal pathogenicity. Current Opinion in Plant Biology. 1:324-326.
Koenning, S. 2001. Soybean Sudden Death Syndrome, Soybean Disease Information Note 7. Plant Pathology Extension, North carolina state university Raleigh, NC.
Kolattukudy, P. E., L. M. Rogers, D. Li, C. S. Hwang and M. A. Flaishman. 1995. Surface signaling in pathogenesis. Proceedings of the National Academy of Sciences USA. 92:4080-4087.
Kosai, N. Mutlak and D. Ali, K. Al-Hiyal. 2010. An Estimation of Demand and Supply Functions for Rice in Iraq for the Period (1980-2005). The Iraqi Journal of Agricultural Sciences. 41 (5):154-169.
Manandhar, T. and K. D. Yami. 2008. Biological control of foot rot disease of rice using fermented products of compost and vermicompost. Scientific World. 6 (6): 52-54.
Marasas, W. F. O., P. E. Nelson and T. A. Toussoun. 1984. Toxigenic Fusarium species, identity and mycotoxicology. The Pennsylvania state.university press.
Mostapha, N. K. 2004.Biological control of Rhizoctonia solani, the causal agent of rice sheath blight by antagonistic bacteria in greenhouse and field conditions. Plant Pathology Journal. 3(2):88-96.
Manamgoda, D. S., L. Cai, E. H. McKenzie, P. W. Crous, H. Madrid, E. Chukeatirote and K. D. Hyde. 2012. A phylogenetic and taxonomic re-evaluation of the Bipolaris Cochliobolus Curvularia Complex. Fungal Diversity. 56 (1): 131-144.
Manamgoda, D. S., A. Y. Rossman, L. A. Castlebury, P. W Crous, H. Madrid, E. Chukeatirote, and K. D. Hyde. 2014. The genus Bipolaris. Studies in Mycology, 79: 221–288.
Maryam, K. D., J. N. Mohammad, M. V. R. Bahar and H. Shahab. 2013. Analysis of the association between Fusarium verticillioides strains isolated from rice and corn in Iran bymolecular methods. European Journal of Experimental Biology. 3:90-96.
Morgavi, D. and R. T. Riley. 2007. An historical overview of field disease outbreaks known or suspected to be caused by consumption of feeds contaminated with Fusarium toxins. Animal Feed Science and Technology. 137: 201-212.
Murray, M. G. and W. F. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.
Najeeb, M., M.Ahmed,S.Bashir, N.A.Bhat, S.K.Maheshwari. 2008. Status of Rice Sheath Blight (Thanatephorus cucumeris) in Kashmir. nnals of Plant Protection Sciences. 16 (2): 508-509.
Nelson, P.E., T. A. Toussoun and W. F. O. Marasas. 1983. Fusarium species: An illustrated manual for identification. The Pennsylvania State University Press.
Nur Ain Izzati, M. Z., M. S. Siti Nordahliawate, J. Nor Azlina, A. A. R. Darnetty and S. Baharuddin. 2011. Isolation and Identification of Fusarium species Associated with Fusarium Ear Rot Disease of Corn. Pertanika Journal ofTropical Agricultural Scienc. 34 (2): 325 – 330.
Oerke, E. C. and H. W. Dehne. 2004. Safeguarding production – losses in major crops and the role of crop protection. Crop Protection 23: 275–285. (http://www.un.org/esa/population/publications/wpp2006/wpp2006.htm).
Roncero, M. G. I., H. Concepcio, M. Ruiz-Rubio, F. I. Garcıa Maceira, M. P. Madrid, F. C. Zaira Caracuel, J. Delgado-Jarana, R. Roldan-Rodrıguez, A. L. Martınez- Rocha, C. V. Juan Roa, Martın-Urdiroz, D. M. Cordoba and A. Di Pietro. 2003. Fusarium as a model for studying virulence in soil borne plant pathogens. Physiological and Molecular Plant Pathology. 62:87–98.
Salih, H. M., W. O. Hussain and M.S. Hassan. 2000. Seed-borne Fungi Associated with some Rice Cultivars Seeds in the Middle of Iraq. The Iraqi Journal of Agricultural Science. 37(6): 105-108.
Sami, A. A. A. 2006. Isolation and Identification of Fungi causing of Brown Leaf spot Disease on Rice in two Governorates Al-Najaf and Al-QadisiahJournal of the University of Karbala scientific. 1: 86-95.
Schwarz, C., C. Tiessen, M. Kreutzer, T. Stark, T. Hofmann and D. Marko. 2012. Characterization of a genotoxic impact compound in Alternaria alternata infested rice as Altertoxin II. Archives of toxicology. 86: 1911-1925.
Shabana, Y. M., Abdel-Fattah, G. M., Ismail, A. E., and Rashad, Y. M. 2008. Control of brown spot pathogen of rice (Bipolaris oryzae) using some phenolic antioxidants. Brazilian Journal of Microbiology. 39: 438-444.
Sharma, K., E. M. Goss, E. R. Dickstein, M. E. Smith, J. A. Johnson, F. S. Southwick and A. H. C. van Bruggen. 2014. Exserohilum rostratum: Characterization of a Cross-Kingdom Pathogen of Plants and Humans. PLoS ONE. 9 (10): e108691.
Sharon, M., S. Kuninaga, M. Hyakumachi, S. Naito and B. Sneh. 2008. Classification of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping. Mycoscience. 49 (2): 93-114.
Sharon, M., B. Sneh, S. Kuninaga and M. Hyakumachi. 2006. The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping. Mycoscience. 47(6): 299-316.
Sheehy, J. E., A. B. Ferrer and P. L. Mitchell. 2008. Harnessing photosynthesis in tomorrow’s world: humans, crop production and poverty alleviation. In Energy from the Sun (Allen, J.F. et al., eds). pp. 1243–1248.
Singh, A., S. Singh, S. Singh, T. Singh, V. Singh, V. Pandey and U. Singh. 2009. Fungal spore germination inhibition by alkaloids dehydrocorydalmine and oxyberberine. Journal of Plant Protection Research. 49(3): 287-289.
Sousa, R. S., F. Á. Rodrigues, D. A. Schurt, N. F. A. Souza and M. F. A. Cruz. 2013. Cytological aspects of the infection process of Pyricularia oryzae on leaves of wheat plants supplied with silicon. Tropical Plant Pathology. 38: 472-477.
Skamnioti, P. and S. J. G. Gurr. 2009. Against the grain: safeguarding ricefrom rice blast disease. Review. (http://ssrn.com/abstract=1149123).
Summerell, B. A. and J. F. Leslie. 2011. Fifty years of Fusarium: how could nine species have ever been enough? Fungal Diversity. 50: 135–144.
Takeo, U. and O. Kaoru. 1990. Participation of Oryza sativa leaf wax in appressorium formation by Pyricularia oryzae. Phytochemistry. 29: 91–92.
Talbot, P. B. B. 1970. Taxonomy and nomenclature of the perfect state. In: Rhizoctonia solani, Biology and Pathology. Edited by Parmeter (JR), J.R. University of California Press. Berkeley, Los Angeles and London. pp. 20-31.
Tan, W., Zhang, W. Ou, Z. Li, C. Zhou,G. Wang, Z.and Yin,L.2007. Analyses of the temporal development and yield losses due to sheath blight of rice (Rhizoctonia solani AG1.1a). Agriculture Science China. 6:1074-108.
Thabet, Y., R. Ali, A. T. Mohamed, G. Kamel and B. Jalel. 2008. Control of dampingoff caused by Rhizoctonia solani and Fusarium solani using olive mill waste water and some of its indigenous bacterial strains. Crop Protection. 27:189–197.
Vidhyasekaran, P., E. S. Borromeo, D. H. Ling and T. W. Mew. 1991. Relationship between growth rate of Helminthosporium oryzae isolates on calluses of rice cultivars and their disease reaction on rice plants. Plant cell, tissue and organ culture. 24: 237-241.
Vijayan, M., C. M. Nair. 1985. Anastomosis group of isolates of Rhizoctonia solani (Thanatephorus cucumeris) causing sheath blight of rice. Current Science. 54: 289-291.
Vincent, W. C. and J. C. Cochrane. 1971. Chlamydospore induction in pure culture in Fusarium solani. Mycologica. 63: 462-477.
White, T. J., T. Bruns, S. Lee, J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J. Academic Press, Inc., NewYork, pp. 315-322.
Zaccardelli, M., S. Vitale, L. Luongo, M. Merighi and L. Corazza. 2008. Morphological and Molecular Characterization of Fusarium solani isolates. Journal Phytopathology. 156: 534–541.
Zhang, L. X., S. S. Li, G. J. Tan, J. T. Shen and T. He. 2012. First Report of Nigrospora oryzae Causing Leaf Spot of Cotton in China. Plant Disease.9:1,379.3 - 1,379.3.
DOI: 10.33687/phytopath.005.01.1328
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Copyright (c) 2016 Hamdia Z. Ali, Hadi M. Aboud, Naeem S. Dheyab, AbdulRahman AbdulQader, Ameera S. Mohammed, Hutham M. Saood, Suraa H. Obiad, Salam D. Salman
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