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Antibacterial Activity of Coptis chinensis Extract Against Pectobacterium carotovorum subsp. carotovorum

Stephen Kironji Githeng'u, Samuel Nyalala, Liu Gaoqiong

Abstract


Soft rot of Zantedeschia, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), has caused a significant worldwide threat to calla lily production. Hence, in order to effectively manage the disease, an intensive management programme aimed at adequte suppression of the pathogen is paramount. In order to investigate the antibacterial effect of a Coptis chinensis extract product against the soft rot- causing bacterium, an in vitro study was set up. Bacterial isolate were obtained from rotting calla lily tubers and maintained in Nutrient Broth under refrigeration. Sterile petri plates containing 15 ml of Nutrient agar were prepared and aseptically inoculated with 0.1 ml an overnight grown culture of a standardized Pectobacterium carotovorum subsp. carotovorum inoculum containing about 1x 108 cfu/ml. The inoculum was spread evenly over the whole surface of the plates. After solidification, 1 ml of the different concentrations of C. chinensis (1, 10, 25, 50 and 100%) were placed individually at the centre of the inoculated petri plates. Positive check was maintained using Streptomycin sulphate (100ppm) and a negative check using sterile distilled water. Eight replicates were maintained for each treatment and the experiment was repeated twice. Results indicated that the lower concentrations of C. chinensis did not cause any inhibition against Pcc. On the other hand, 100% C. chinensis made an inhibition zone comparable to that streptomycin sulphate. Our results demonstrated that C. chinensis has antibacterial activity and therefore feasible for use in crop protection against soft rots caused by Pectobacterium carotovorum subsp. carotovorum.

Keywords


Nutrient Agar; inhibition zone; soft rot; inoculum

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DOI: 10.33687/phytopath.005.02.1159

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