Survey and Genetic Diversity of Grapevine Leafroll Associated Virus 2 in Algeria

Arezki Lehad, Ilhem Selmi, Meriem Louanchi, Mouni Aitouada, Naima Mahfoudhi


Vineyards in western and center regions of Algeria were surveyed for the Grapevine leafroll-associated virus 2 (GLRaV-2). Analyses by DAS-ELISA and Reverse Transcription Polymerase Chain Reaction (RT-PCR) reveal 15, 8% prevalence. The genetic diversity of the GLRaV-2 population was studied by phylogenetic analyses of the HSP70h gene region of seven samples sequenced in this study and other sequences downloaded from GenBank. Results reveal segregation of the GLRav-2 population into six distinct groups. An estimation of the ratio of non-synonymous substitutions per non-synonymous site to synonymous substitutions per synonymous site indicated that HSP70h gene evolve under positive selection. Similarity plot constructed with representative sequence from each group confirmed previous results.  All Algerian isolates belong to group PN. As far as we know, this is the first characterization of GLRaV-2 isolates from Algeria.


GLRaV-2; RT-PCR; genetic diversity; HSP70h gene


Abou-Ghanem, N. and S. Sabanadzovic and A. Minafra and P. Saldarelli and G. Martelli. 1998. Some properties of Grapevine leafroll-associated virus 2 and molecular organization of the 3'region of the viral genome. Journal of Plant Pathology. 37-46.

Angelini, E. and N. Bertazzon and M. Borgo. 2004. Diversity among Grapevine Leafroll‐associated Virus 2 Isolates Detected by Heteroduplex Mobility Assay. Journal of Phytopathology. 152: 416-22.

Bertazzon, N. and M. Borgo and S. Vanin and E. Angelini. 2010. Genetic variability and pathological properties of Grapevine leafroll-associated virus 2 isolates. European Journal of Plant Pathology. 127: 185-97.

Beuve, M. and L. Sempé and O. Lemaire. 2007. A sensitive one-step real-time RT-PCR method for detecting Grapevine leafroll-associated virus 2 variants in grapevine. Journal of Virological Methods. 141: 117-24.

Clark, M. F. and A. Adams. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of general virology. 34: 475-83.

Ghanem-Sabanadzovic, N. A. and S. Sabanadzovic and M. Castellano and D. Boscia and G. Martelli. 2000. Properties of a new isolate of Grapevine leafroll-associated virus 2. VITIS-GEILWEILERHOF-. 39: 119-22.

Goszczynski, D. and G. Kasdorf and G. Pietersen and H. Van Tonder. 1996. Detection of two strains of grapevine leafroll-associated virus 2. VITIS-GEILWEILERHOF-. 35: 133-6.

Greif, C. and R. Garau and D. Boscia, et al. 1995. The relationship of grapevine leafroll-associated closterovirus 2 with a graft incompatibility condition of grapevines. Phytopathologia Mediterranea. 167-73.

Gugerli, P. and J. Brugger and R. Bovey. 1984. L'enroulement de la vigne: mise en evidence de particules virales et developpement d'une methode immuno-enzymatique pour le diagnostic rapide. Revue suisse de viticulture, arboriculture, horticulture.

Jarugula, S. and O. J. Alabi and R. R. Martin and R. A. Naidu. 2010. Genetic variability of natural populations of Grapevine leafroll-associated virus 2 in Pacific Northwest vineyards. Phytopathology. 100: 698-707.

Karasev, A. V. 2000. Genetic diversity and evolution of closteroviruses. Annual review of phytopathology. 38: 293-324.

Lekikot, A. K., Elbeaino, T., Ghezli, C., and Digiaro, M. 2012. Proceedings of the 17th Congress of the International Council for the Study of Preliminary Survey of Grapevine Viruses in Algeria, Davis, California, USA, October 7-14, 2012. University of California, Foundation Plant Services.

Martelli, G. and N. Abou Ghanem-Sabanadzovic and A. Agranovsky, et al. 2012. Taxonomic revision of the family Closteroviridae with special reference to the grapevine leafroll-associated members of the genus Ampelovirus and the putative species unassigned to the family. Journal of Plant Pathology. 94: 7-19.

Martelli, G. P. 2014. Directory of virus and virus-like diseases of the grapevine and their agents. Journal of Plant Pathology. 96: S1-S136.

Meng, B. and C. Li and D. E. Goszczynski and D. Gonsalves. 2005. Genome sequences and structures of two biologically distinct strains of Grapevine leafroll-associated virus 2 and sequence analysis. Virus genes. 31: 31-41.

Moury, B. and V. Simon. 2011. dN/dS-based methods detect positive selection linked to trade-offs between different fitness traits in the coat protein of potato virus Y. Molecular biology and evolution. 28: 2707-17.

Rowhani, A. and Y. Zhang and D. Golino and J. Uyemoto. 2000. Isolation and partial characterization of two new viruses from grapevine. Proceedings of XIII International Council for the Study of Viruses and Virus-Like Diseases of the Grapevine, Adelaide. 82.

Rowhani, A. and Y. Zhang and D. Golino and J. Uyemoto. 2002. Isolation and characterization of a new closterovirus from grapevine. Phytopathology. 92: S71.

Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution. 4: 406-25.

Svanella-Dumas, L. and M. Dulucq and T. Candresse and P. Gentit and X. Foissac. Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). Proceedings of the XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops-Top Fruit Diseases 550, 2000, 37-44.

Tamura, K. and G. Stecher and D. Peterson and A. Filipski and S. Kumar. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution. 30: 2725-9.

Thompson, J. D. and D. G. Higgins and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research. 22: 4673-80.

Zhu, H. and K. Ling and D. Goszczynski and J. Mcferson and D. Gonsalves. 1998. Nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 are similar to beet yellows virus, the closterovirus type member. Journal of general virology. 79: 1289-98.

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DOI: 10.33687/phytopath.004.01.1074


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