Evaluation of chemical treatments for managing bacterial leaf spot in chili caused by Xanthomonas campestris pv. vesicatoria

Tahreem Fatima, Muhammad Atiq, Luqman Aamrao, Muhammad Muzammil Jahangir, Jamil Shafi, Ahmad Nawaz, Muhammad Jahanzaib Matloob, Asad Ullah, Nasar ur Rehman Khan, Aniqa Ijaz, Hadeeqa Riasat

Abstract


Chilli, a highly important and valuable crop worldwide, belongs to the Solanaceae family. In Pakistan, Sindh is the leading producer of chilli. However, bacterial leaf spot (BLS) caused by Xanthomonas campestris pv. vesicatoria poses a significant threat, leading to yield losses of up to 40% globally. Effective management of BLS has become a critical concern. In the current study, five chemicals, Cabrio Top, Oxyrich, Kocide, Electus Super, and Forum Top, were evaluated for their efficacy in managing BLS. An in vitro experiment was conducted using a completely randomized design with three replications per treatment and three concentrations (200, 250, and 300 ppm) of each chemical. The results revealed that Cabrio Top exhibited the largest inhibition zone (18.710 mm), followed by Electus Super (16.730 mm), Forum Top (13.653 mm), and Kocide (11.759 mm), compared to the untreated control. Subsequently, an in vivo experiment was designed using the randomized complete block design with three replications and three concentrations (1, 2, and 3 g/L) of the best-performing chemicals. The combined treatment of Cabrio Top + Oxyrich demonstrated the most significant results, showing the lowest disease incidence (23.544%), followed by Oxyrich (31.559%) and Cabrio Top (37.193%). The findings of this study conclude that the combined application of Cabrio Top and Oxyrich is a promising approach for managing BLS in chilli under field conditions.


Keywords


Cabrio Top, Chili bacterial diseases, Chemical control strategies, Bacterial leaf spot management, Disease suppression

Full Text:

PDF

References


Abrahamian, P., Jones, J.B., Vallad, G.E., 2019. Efficacy of copper and copper alternatives for management of bacterial spot on tomato under transplant and field production. Jounalof Crop Protection 126,104919.

Aftab, Z.-E.-H., Aslam,W., Aftab, A., Shah, A.N., Akhter, A., Fakhar, U., Siddiqui, I., Ahmed, W., Majid F., Wróbel, J., 2022. Incorporation of engineered nanoparticles of biochar and fly ash against bacterial leaf spot of pepper. Scientific Reports 12, 1-13.

Arin, S., 2019. Scenario of chilli production and hindrances faced by the growers of Sindh province of Pakistan. Modern Concepts & Developments in Agronomy 4, 436-442.

Asghar, A., Mukhtar, T., Raja, M.U., Gulzar, A., 2020. Interaction between Meloidogyne javanica and Ralstonia solanacearum in chili. Pakistan Journal of Zoology 52(4), 1525-1530.

Ashfaq, M., Iqbal, S., Mukhtar, T., Shah, H., 2014. Screening for resistance to cucumber mosaic cucumovirus in chilli pepper. Journal of Animal and Plant Sciences 24(3), 791-795.

Aslam, M.N., Mukhtar T., 2024. Evaluation of virulence among Pakistani isolates of Ralstonia solanacearum inducing bacterial wilt in chilies across different agro-ecological zones. Bragantia 83, e20230181.

Aslam, M.N., Mukhtar, T., 2023a. Distributional spectrum of bacterial wilt of chili incited by Ralstonia solanacearum in Pakistan. Bragantia 82, e20220196.

Aslam, M.N., Mukhtar, T., 2023b. Characterization of Ralstonia solanacearum causing bacterial wilt from major chili growing areas of Pakistan. Bragantia 82, e20230001.

Aslam, M.N., Mukhtar, T., Ashfaq, M., Hussain, M.A., 2017. Evaluation of chili germplasm for resistance to bacterial wilt caused by Ralstonia solanacearum. Australasian Plant Pathology 46(3), 289-292.

Aziz, S., Jamshed, S.A., Mukhtar, T., Irshad, G., Ijaz, S.S., Raja, M.U., 2024. Evaluation of Bacillus spp. as biocontrol agents against chili leaf spot caused by Xanthomonas vesicatoria. Journal of Plant Diseases and Protection 131, 987-997.

Bhaik, A., Mboup, M.K., Genet, J.-L., 2022. Fungicide Resistance: Threats and Management Approaches. Sustainable Management of Potato Pests and Diseases. Springer 59-81.

Gambley, C., 2018. Management and detection of bacterial leaf spot in capsicum and chili crops. Hort Innovation 2, 20.

GOP, 2021. Agricultural Statistics of Pakistan. Pakistan Bureau of Statistics, Agriculture Statistics Section, Islamabad.

Hameed, A., Atiq, M., Rajput, N.A., Alsamadany, H., Alzahrani, Y., Sahi, S.T., Shah, Z.H., Sarfraz, S., Liaqat, H., Tariq, H., Afzal, A., 2020. Role of mineral nutrients in confronting citrus canker caused by Xanthomonas axonopodis pv. citri. International Journal of Biosciences 17, 113-119.

Hassan, E.O., Zyton, M.A., 2017. Management of bacterial spot of pepper caused by Xanthomonas campestris pv. vesicatoria. American Journal of Bioscience and Bioengineeringat 5, 41-49.

Iqbal, U., Mukhtar, T., 2020. Inhibitory effects of some fungicides against Macrophomina phaseolina causing charcoal rot. Pakistan Journal of Zoology 52(2), 709-715.

Islam, M.H., Islam, S., Masud, M.M., Mita, M.M., Islam M.S., Islam, M.R., 2022. Identification of potential chemical fungicides with diverse groups of active ingredient for controlling late blight of potato in Bangladesh. Asian-Australasian Journal of Bioscience and Biotechnology 7, 23-35.

Khanal, S., Hind S.R., Babadoost, M., 2020. Occurrence of copper-resistant Xanthomonas perforans and X. gardneri in Illinois tomato fields. Plant Health Progress 21, 338-344.

Kumar, N., Satbhai, A.K., Ramalingam, L., Schmitt, C., Moustaid-Moussa, N., Crago, J., 2020. Developmental toxicity in embryo-larval zebrafish (Danio rerio) exposed to strobilurin fungicides (azoxystrobin and pyraclostrobin). Chemosphere 241, 124980.

Majumdar, S., Long, R.W., Kirkwood, J.S., Minakova, A.S., Keller, A.A., 2021. Unraveling metabolic and proteomic features in soybean plants in response to copper hydroxide nanowires compared to a commercial fertilizer. Environmental Science & Technology 55(20), 13477-13489.

Mincă, D., Mitrea, R., 2020. The use of uncertified seeds-common but dangerous practice that promotes the spred of Xanthomonas campestris pv. vesicatoria (doidge) dye. Current Trends in Natural Sciences 9(17), 31-37.

Monteiro, G.C., Goto, R., Minatel, I.O., de Sousa da Silva, E., da Silva, E.G., Vianello, F., Lima, G.P.P., 2019. Grafting, agrochemicals, and oxidative enzymes as factor for plant biotic resistance. Plant Health Under Biotic Stress: Organic Strategies 37-57.

Olaes, E.J., Arboleda, E.R., Dioses, J.L., Dellosa, R.M., 2020. Bell pepper and chili pepper classification: An application of image processing and fuzzy logic. International Journal of Scientific & Technology Research 9(2), 4832-4839.

Otten, C., Büttner, D., 2021. HrpB4 from Xanthomonas campestris pv. vesicatoria acts similarly to SctK proteins and promotes the docking of the predicted sorting platform to the type III secretion system. Cellular Microbiology 23(6), e13327.

Pereira, J., King, A., Campos, M.G., Santra, S., 2022. Advanced copper and copper alternatives for crop protection-a mini-review. Current Nanoscience 18(4), 410-424.

Poudel, N.S., Neupane, S., 2018. Bacterial diseases of plants in Nepal: A review. Asian Journal of Agricultural and Horticultural Research 2(1), 1-10.

Rajwade, J.M., Chikte, R.C., Singh, N., Paknikar, K.M., 2022. Copper-based nanostructures: Antimicrobial properties against agri-food pathogens. In Copper nanostructures: Next-generation of agrochemicals for sustainable agroecosystems. Elsevier 477-503.

Rekanović, E., Potočnik, I., Stepanović, M., Todorović, B., Mihajlović, M., Milijašević-Marčić, S., 2019. Control of pepper bacterial spot and tomato bacterial speck using bactericides in combination with a plant activator. Pesticidi i fitomedicina 34(1), 47-52.

Roach, R., Mann, R., Gambley, C.G., Shivas, R.G., Chapman, T., Rodoni, B., 2020. Pathogenicity and copper tolerance in Australian Xanthomonas species associated with bacterial leaf spot. Crop Protection, 127, 104923.

Roach, R., Mann, R., Gambley, C.G., Shivas, R.G., Rodoni, B., 2018. Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia. European Journal of Plant Pathology 150, 595-608.

Roylawar, P., Khandagale, K., Randive, P., Shinde, B., Murumkar, C., Ade, A., Singh, M., Gawande, S., Morelli, M., 2021. Piriformospora indica primes onion response against stemphylium leaf blight disease. Pathogens 10(9),1085.

Saba, S., Mukhtar, T., Haq, M.I., Malik, S.I., 2022. Occurrence of damping off of chili caused by Pythium spp. in the Pothwar region of Pakistan. International Journal of Phytopathology 11(2), 125-234.

Saeed, M., Mukhtar, T., 2024. Root-knot nematodes (Meloidogyne spp.) infecting peach (Prunus persica L.) in the Pothwar region of Pakistan. Journal of Agricultural Science and Technology 26(4), 897-908.

Saeed, M., Mukhtar, T., Ahmed, R., Ahmad, T., Iqbal, M.A., 2023. Suppression of Meloidogyne javanica infection in peach (Prunus persica (L.) Batsch) using fungal biocontrol agents. Sustainability 15(18), 13833.

Saha, P., Bera, S., 2019. Bioefficacy and phytotoxicity evaluation of Metiram 70% WG: A new fungicide molecule against anthracnose disease of chilli caused by Colletotrichum capsici. Indian Journal of Plant Protection 47(3&4), 164-172.

Selim, R.E., Khalil, M.S., 2021. Strobilurins: New group of fungicides. Journal of Plant Science and Phytopathology 5(2), 63-064.

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, Obradović, A., 2019. Integration of biological and conventional treatments in control of pepper bacterial spot. Crop Protection 119, 46-51.

Shahbaz, M.U., Mukhtar, T., Haque, M.I., Begum, N., 2015. Biochemical and serological characterization of Ralstonia solanacearum associated with chilli seeds from Pakistan. International Journal of Agriculture and Biology 17(1), 31-40.

Sharma, A., Kumar, M., Dogra, R.K., Kumar, N., Kumari, R., Kansal, S., 2019. Estimation of genetic variability in bell pepper (Capsicum annuum L. var. grossum). International Journal of Chemical Studies 7(3), 10-13.

Sharma, R., Gaur, R., 2021. Controlling canker and stem-end rot of Kinnow (Citrus deliciosa Ten.). The Pharma Innovation Journal 10(3), 459-462.

Sharma, R.K., Patel, J.K., Patel, D.R., Patel, R.N., 2020. Management of early blight of potato (Solanum tuberosum L.) caused by Alternaria solani [(Ellis & Martin) Jones & Grout] through fungicides and its impact on yield. International Journal of Current Microbiology and Applied Sciences 9(3), 1683-1693.

Sieiro-Sampedro, T., Briz-Cid, N., Pose-Juan, E., Figueiredo-González, M., González-Barreiro, C., Simal-Gándara, J., Cancho-Grande, B., Rial-Otero, R., 2020. Tetraconazole alters the methionine and ergosterol biosynthesis pathways in Saccharomyces yeasts promoting changes on volatile derived compounds. Food Research International 130, 108930.

Singh, A.P., Singh, R.S., Singh, S.R.P., Pal, M., Singh, R., Kumari, R., 2021. Screening of genotypes against major biotic stresses in chilli (Capsicum annum L.). The Pharma Innovation Journal 10(2), 502-505.

Stahle, L., Wold, S., 1989. Analysis of variance (ANOVA). Chemometrics and intelligent laboratory systems 6(4), 259-272.

Tahir, H.A., Sahi, S.T., Habib, A., Haq, I.U., Ahmad, A., Ashraf, W., 2016. Evaluation of plant extracts as biocontrol agents against Xanthomonas axonopodis pv. citri the cause of citrus canker. Pakistan Journal of Phytopathology, 28(1): 35-43.

Tooley, L.J., 2019. Whole-plant corn silage and foliar fungicide: Strategies to improve dairy feed quality and profitability (Doctoral dissertation, University of Illinois at Urbana-Champaign). http://hdl.handle.net/2142/106404

Tripathi, V., Jeshwin Giftson, S.P., Pooja, M., Pranao, S., Goutham, G.S., Amjith Mohan, M.L., 2022. Value addition in horticultural crops. The Pharma Innovation Journal 11(5S), 1058-1067.

Van Bruggen, A.H.C., Finckh, M.R., 2016. Plant diseases and management approaches in organic farming systems. Annual Review of Phytopathology 54(1), 25-54.

Yang, L.C., Gan, Y.L., Yang, L.Y., Jiang, B.L., Tang, J.L., 2018. Peptidoglycan hydrolysis mediated by the amidase AmiC and its LytM activator NlpD is critical for cell separation and virulence in the phytopathogen Xanthomonas campestris. Molecular Plant Pathology 19(7),1705-1718.

Yaseen, I., Mukhtar, T., Kim, H.T., Arshad, B., 2023. Quantification of resistance to Melidogyne incognita in okra cultivars using linear and nonlinear analyses of growth parameters and nematode infestations. Bragantia 82, e20230114.

Yaseen, I., Mukhtar, T., Kim, H.-T., Arshad, B., 2024. Interactive effects of Meloidogyne incognita and Fusarium oxysporum f.sp. vasinfectum on okra cultivars. Bragantia 83, e20230266.

Younas, M., Atiq, M., Rajput, N.A., Abbas, W., Rizwan Bashir, M., Ahmad, S., Sami Ullah, M., Ashraf Bhatti, W., Liaqat, N., Ahmad, I., 2021. Induction of resistance in onion against purple leaf blotch disease through chemicals. Asian Journal of Agriculture and Biology 3, 1-7.




DOI: https://doi.org/10.33804/pp.008.04.5409

Refbacks

  • There are currently no refbacks.