Appraisement of chemotherapy, plant defense activators, and genetic resistance against eyespot disease in sugarcane

Fahad Yaqoob, Muhammad Atiq, Nasir Ahmed Rajput, Ahmad Nawaz, Muhammad Kashif, Muhammad Jahanzaib Matloob, Abdul Jabbar, Wasi -ud-Din, Farhan Ali, Asad Ullah

Abstract


Sugarcane (Saccharum officinarum) is a crucial agro-industrial crop that faces numerous biotic and abiotic challenges. One of the most severe biotic stresses affecting sugarcane is eyespot disease, caused by Bipolaris sacchari. This study focused on managing eyespot disease using resistant varieties, chemicals, and plant defense activators. Disease samples were collected from Lalian, Chiniot, and Faisalabad, and the pathogen was isolated and identified from the infected leaves. In the screening experiment, 10 sugarcane varieties were evaluated. CPF-246 exhibited high resistance, while CPF-247 and CPF-77400 showed resistance to eyespot disease. The poisoned food technique was used to assess fungicides at different concentrations (50, 100, and 150 ppm) under in vitro conditions. Tilt (Propiconazole) exhibited the maximum growth inhibition (4.645 mm) under these conditions. Plant activators were tested at different concentrations (0.25%, 0.5%, and 0.75%) in greenhouse conditions. Salicylic acid resulted in the minimum disease incidence (13.236%, 7.483%, and 6.473%) after 15, 30, and 45 days, respectively. Under field conditions, the combination of Salicylic acid and Tilt (12.395%) showed the lowest disease incidence. By employing these newly investigated management strategies, the farming community can effectively combat the significant threat posed by eyespot disease to the sugarcane industry.


Keywords


Eyespot Disease; Chemotherapy; Plant Defense Activators; Genetic Resistance; Bipolaris sacchari; Disease management.

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References


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DOI: https://doi.org/10.33804/pp.008.02.5182

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