Chemical Profile and Antifungal Activity of Leaf Extract of Tabernaemontana divaricata against Macrophomina phaseolina

Arshad Javaid, Iqra Haider Khan


Macrophomina phaseolina is a highly problematic fungus that causes diseases in hundreds of plant species. In order to search for an alternative to chemical fungicides for the control of this devastating pathogen, leaf extract of Tabernaemontana divaricata was assessed for the control of M. phaseolina. Five concentrations of leaf extract in methanol viz. 1, 2, 3, 4 and 5% (w/v), were checked against M. phaseolina. All these concentrations significantly suppressed the fungal growth resulting in 34–74% decreased biomass of M. phaseolina over control. GC-MS analysis of this extract showed the presence of 54 compounds. Squalene was the predominant compound with 24.11% peak area followed by vitamin E (8.96%). Other important compounds were hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (6.21%), thiophene, tetrahydro-2-methyl- (5.68%), cyclopentanol (5.61%), neophytadiene (6.25%), 1-tert-butoxypropan-2-yl 2-methylbutanoate (6.03%), ibogamine-18-carboxylic acid, 12-methoxy-, methyl ester (2.95%), phytol (2.26%), and n-hexadecanoic acid (2.20%). Some of these major compounds might be responsible for antifungal property of methanolic leaf extract of T. divaricata against M. phaseolina.


Antifungal; Leaf extract; Macrophomina phaseolina; Tabernaemontana divericata

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Amin, A., Akbar, M., Khalil, T., Akram, W., Ahmad, A., 2022. Antifungal activity of Alternanthera philoxeroides organic solvent extracts against plant pathogenic fungi. Pakistan Journal of Botany 54(1), 337-344.

Banaras, S., Javaid, A., Khan, I.H., 2020. Potential antifungal constituents of Sonchus oleraceous against Macrophomina phaseolina. International Journal of Agriculture and Biology 24(5), 1376-1382.

Banaras, S., Javaid, A., Khan, I.H., 2021. Bioassays guided fractionation of Ageratum conyzoides extract for the identification of natural antifungal compounds against Macrophomina phaseolina. International Journal of Agriculture and Biology 25(4), 761-767.

Boligon, A.A., Piana, M., Kubiça, T.F., Mario, D.N., Dalmolin, T.V., Bonez, P.C., Weiblen, R., Lovato, L.T., Alves, S.H., Campos, M.M., 2015. HPLC analysis and antimicrobial, antimycobacterial and antiviral activities of Tabernaemontana catharinensis A. DC. Journal of Applied Biomedicine 13, 7-18.

Dordab, T., Sourinejad, I., Nazemi M., 2021. Antibacterial effect of the squalene extracted from the liver of the Persian Gulf spot tail shark Carcharhinus sorrah (Müller & Henle, 1839). Journal of Fisheries Science and Technology 10(2), 251-258.

Ferdosi, M.F.H., Ahmed, H., Khan, I.H., Javaid, A., 2022. Fungicidal potential of flower extract of Cassia fistula against Macrophomina phaseolina and Sclerotium rolfsii. Journal of Animal and Plant Sciences 32(4), 1028-1034.

Ferdosi, M.F.H., Khan, I.H., Javaid, A., Nadeem, M., Munir, A. 2021. Biochemical profile of Calotropis procera flowers. Pakistan Journal of Weed Science Research 27(3), 341-349.

Ghaneian, M.T., Ehrampoush, M.H., Jebali, A., Hekmatim, O., Mahmoudi, M., 2015. Antimicrobial activity, toxicity and stability of phytol as a novel surface disinfectant. Environmental Health Engineering and Management Journal 2, 13-16.

Huang, Z.R., Lin Y.K., Fang, J.Y. 2009. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules 14, 540-54.

Hyder, S., Sahi, S.T., Gondal, A.S., Hannan, A., Rehman, A., Ahmed, R., 2018. First report of tomato charcoal rot caused by Macrophomina phaseolina (Tassi) Goid from Pakistan. Plant Disease 102(7), 1459.

Iqbal, U., Mukhtar, T., 2014. Morphological and pathogenic variability among Macrophomina phaseolina isolates associated with mungbean (Vigna radiata L.) Wilczek from Pakistan. The Scientific World Journal 2014, Article ID 950175, 9 pages.

Iqbal, U., Mukhtar, T., 2020a. Inhibitory effects of some fungicides against Macrophomina phaseolina causing charcoal rot. Pakistan Journal of Zoology 52(2): 709-715.

Iqbal, U., Mukhtar, T., 2020b. Evaluation of biocontrol potential of seven indigenous Trichoderma species against charcoal rot causing fungus, Macrophomina phaseolina. Gesunde Pflanzen, 72(2), 195–202.

Iqbal, U., Mukhtar, T., Iqbal, S.M., 2014. In vitro and in vivo evaluation of antifungal activities of some antagonistic plants against charcoal rot causing fungus, Macrophomina phaseolina. Pakistan Journal of Agricultural Sciences 51 (3): 689-694.

Javaid, A., Khan, I.H., Ferdosi, M.F.H., 2021. Bioactive constituents of wild Cannabis sativa roots from Pakistan. Pakistan Journal of Weed Science Research 27(3), 359-368.

Javaid, A., Khan, I.H., Shoaib, A., 2018. Management of charcoal rot of mungbean by two Trichoderma species and dry biomass of Coronopus didymus. Planta Daninha 36, Article e018182795.

Javed, S., Mahmood, Z., Khan, K.M., Sarker, S.D., Javaid, A., Khan, I.H., Shoaib, A., 2021. Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina. Scientific Reports 11, 8417.

Kaur, S., Dhillon, G.S., Brar, S.K., Vallad, G.E., Chand, R., Chauhan, V.B., 2012. Biology, economic importance and current diagnostic trends. Critical Review in Microbiology 38, 136-151.

Khan, I.H., Javaid, A., 2020. Antifungal activity and GC-MS analysis of n-butanol extract of quinoa (Chenopodium quinoa Willd.) leaves. Bangladesh Journal of Botany 49(4), 1045-1051.

Khan, I.H., Javaid, A., 2021. In vitro screening of Aspergillus spp. for their biocontrol potential against Macrophomina phaseolina. Journal of Plant Pathology 103(4), 1195-1205.

Khan, I.H., Javaid, A., 2022a. DNA cleavage of the fungal pathogen and production of antifungal compounds are the possible mechanisms of action of biocontrol agent Penicillium italicum against Macrophomina phaseolina. Mycologia 114(1), 24-34.

Khan, I.H., Javaid, A., 2022b. Histopathological changes in root and stem of mungbean exposed to Macrophomina phaseolina and dry biomass of Chenopodium quinoa. Microscopy Research and Technique 85(7), 2596-2606.

Khan, I.H., Javaid, A., 2022c. Hexane soluble bioactive components of leaf extract of quinoa. Journal of Animal and Plant Sciences 32(2), 309-314.

Lalitha, S., Parthipan, B., Mohan, V.R., 2015. Determination of bioactive components of Psychotria nilgiriensis Deb & Gang (Rubiaceae) by GC-MS analysis. International Journal of Pharmacognosy and Phytochemical Research 7, 802-809.

Mabkhot, Y.N., Alatibi, F., El-Sayed, N.N.E., Bawazeer, S, Hadda, T.B., 2016. Antimicrobial activity of some novel armed thiophene derivatives and petra/osiris/molinspiration (POM) analyses. Molecules 21, 222.

Marquez, N., Giachero, M.L., Declerck, S., Ducasse, D.A., 2021. Macrophomina phaseolina: General characteristics of pathogenicity and methods of control. Frontiers in Plant Sciences 12, Article 634397.

Mikami, Y., YazawaUno, K.J., Matsumae, A., 1990. In vitro activity of amphotericin B, flucytosine, fluconazole and miconazole against clinically isolated Candida albicans, Aspergillus funigatus and Trichosporon beigelii. Chemotherapy 38, 1039-1047.

Prasath, K.G., Tharani, H., Kumar, M.S. Pandian, S.K., 2020. Palmitic Acid Inhibits the Virulence Factors of Candida tropicalis: Biofilms, Cell Surface Hydrophobicity, Ergosterol Biosynthesis, and Enzymatic Activity. Frontiers in Microbiology.

Rahman, M., Islam, M.S., Ali, M.S., Islam, R., Hossain, Z., 2011. Antidiabetic and cytotoxic activities of methanolic extract of Tabernaemontana divaricata (L.) flowers. International Journal of Drug Development & Research 3, 270-276.

Satyanarayan, D., Joshi, A.B., Chandrasekhar, K.S., Vijaynarayan, K., 2004. Anti- inflammatory activity of the flowers of T. divaricata (L.) R.Br. Indian Drugs 41, 405- 409.

Singh, B., Sharma, R.A., Vyas, G.K., 2011. Antimicrobial, antineoplastic and cytotoxic activities of indole alkaloids from Tabernaemontana divaricata (L.) R.Br. Current Pharmaceutical Analysis 7, 125-132.

Uroos, M., Javaid, A., Bashir, A., Tariq, J., Khan, I.H., Naz, S., Fatima, S., Sultan, M., 2022. Green synthesis of coumarin derivatives using bronsted acidic pyridinium based ionic liquid [MBSPy][HSO4] to control an opportunistic human and a devastating plant pathogenic fungus Macrophemina phaseolina. RSC Advances 12, 23963-23972.



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