MOLECULAR IDENTIFICATION AND GROWTH OF XENORHABDUS AND PHOTORHABDUS SYMBIONTS OF ENTOMOPATHOGENIC NEMATODES

Ali Murad rahoo, Rehana Kanwal Rahoo, Muhammad Saeed, Muhammad Burhan, Nusrat Keerio

Abstract


From the characterisation, the partial 16 S gene sequences obtained for the two bacteria were subjected to blast-bootstrap analysis to obtain the phylogenetic tree. In assessing the similarity of Xenorhabdus bovienii with five other Xenorhabdus spp., it was found to be 96% similar to X. nematophila and X. japonica with Accession Numbers D78006 and NR027194 respectively. The X. bovienii was closer to X. beddingii 95% and X. kazodoii and 97% similarity to X. poinarii. From the phylogenetic tree, the two species of bacteria were found to belong to the genera Xenorhabadus and Photorhabdus. The two bacteria were compared with Xenorhabdus japonica NR027194, X. nematophila, D78006 X. poinarii DQ211703, X. beddingii AY278675 and X. kozodoii Eu 190977. The results showed that at 20 ℃ both the bacteria X. bovienii and Photorhabdus luminescens increased to a log concentration of 7.5 after 6 h. They then increased slightly up to 15 h. At 25 ℃ X. bovienii had a concentration of 6.1 after 1 h but only increased slowly to 6.6 after 15 h. However, P. luminescens started with 5.6 after 1 h but increased smoothly to 7.5 after 15 h. X. bovienii and P. luminescens at 30 ℃ had a concentration of 6.2 after 1 h.


Keywords


Xenorhabdus bovienii; Photorhabdus luminescens; growth; molecular identification; Phylogeny

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References


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DOI: https://doi.org/10.33804/pp.006.02.4211

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