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Efficacy of new chemistry insecticides on the haemocytes of maize stem borer, Chilo partellus, under laboratory conditions

Saeed Alam, Muhammad Tariq, Asim Gulzar, Rabia Ali, Shanza Khan, Mubeen Iqbal

Abstract


In the present study, four new chemistry insecticides (Radiant, Chlorfenapyr, Belt and Match) were used to evaluate the haemocytological activity of Chilo partellus. LC50 and LC60 values of each insecticide were calculated by bioassay test. After the application of Radiant insecticide, the total haemocytes count immediately increased (78548.5 cells/mm3) as compared to normal (71255 cells/mm3), however, after 30 minutes decreased to (71694 cells/mm3) and after 60 minutes of interval, the total haemocytes count again increased (80215 cell/mm3). Percent plasmotcytes increased from untreated to 26%, while the spherulocytes, granulocytes, prohaemocytes and cystocytes also fluctuated from the untreated as 26%, 6.75%, 33% and 3.75% respectively. After the application of Chlorfenpyr insecticide, the total Haemocytes count immediately increased (80920.75 cells/mm3) as compared to normal (71255 cells/mm3), however after 30 minutes decreased to (71155 cells/mm3) and after 60 minutes, the total haemocytes count again increased (84100 cell/mm3). Percent plasmotcytes increased from untreated to 36%, while the spherulocytes, granulocytes, prohaemocytes and cystocytes also fluctuated from the untreated to 23.5%, 5.5%, 29% and 1.5% respectively. After the application of Belt insecticide, the total haemocytes count immediately increased (87200 cells/mm3) as compared to normal (71255 cells/mm3) and after 30 minutes decreased (71270 cells/mm3) and after 60 minutes, the total haemocytes count again increased (89640 cell/mm3). Percent plasmotcytes increased from untreated to 36%, while the spherulocytes, granulocytes, prohaemocytes and cystocytes also fluctuated from the untreated to 32.5%, 8.5%, 17.25% and 4.75% respectively. After the application of Match insecticide, the total haemocytes count immediately increased (87500 cells/mm3) as compared to normal (72155 cells/mm3) while after 30 minutes decreased (72190 cells/mm3) and after 60 minutes, the total haemocytes count again increased (99800 cell/mm3). Percent plasmotcytes increased from untreated to 33%, while the spherulocytes, granulocytes, prohaemocytes and cystocytes also fluctuated from the untreated to 31%, 14%, 23% and 4% respectively.


Keywords


Chilo partellus; Haemocytes; Belt; Match; Radiant; Chlorfenpyr

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References


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