Lab-Scale Optimization of Polyhydroxyalkanoate Production by Bacterial Strain cmg1415 on Local Cheap Substrates Using One Variable at a Time Approach

Dr. Muhammadi, Shabina Shafiq

Abstract


Production of polyhydroxyalkanoate (PHA) under optimum culture conditions using local cheap feedstocks is indispensable to overcome the current cost of PHA-based plastics. For this purpose, optimum culture conditions and cheap feedstocks were investigated to produce maximum yield of PHA in CMG1415. Maximum yield was obtained with sucrose or sugar beet as sole source of precursors for PHA in 8 days of incubation at 35 °C in a minimal medium adjusted at pH 7. Further, for maximum yield no mechanical shaking was needed. Local cheap feedstock such as sugar beet and molasses were found to play as significant carbon and nitrogen sources for maximum PHA yield.  Bacterial plastic produced under these low-labor-cost culture conditions may to reduce the present cost of degradable bioplastic and be much effective alternate of nondegradable varieties of synthetic plastic.

Keywords


Culture conditions; Optimization; Local cheap feedstocks

Full Text:

PDF

References


Anderson, A.J and E.A. Dawes. (1990). Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev. 54: 450-472.

Berger E., B.A. Ramsay, J.A. Ramsay and C. Chavarie. (1989). PHB recovery by hypochlorite digestion of non-PHB biomass. Biotechnol Techn. 3: 227-232.

Burdon K.L. (1946). Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparartion. J Bacteriol. 52: 665-678.

Choi, J., F. Wang and S.Y. Lee. (1997). "Production of poly (3-hydroxybutyrate) with high productivity and high polymer content by fed-batch culture of Alcaligenes latus under nitrogen limitation", The 10th Daejeon/Chungnam-Kyushu Symposium on Chemical Engineering, KAIST, Teajon.

Gerrit, E., P. Waard and G.N.M. Huijberts. (1995). Formation of novel poly (hydrooxyalkanoates) from long-chain fatty acids. Can J Microbiol. 41(1): 14-21.

Jendrossek, D., A. Schirmer and H.G. Schlegel. (1996). Biodegradation of polyhydroxyalkanoic acids. Appl Microbial Biotechnol. 46: 451-463.

Kim, Y.B., R.W. Lenz and R.C. Fuller. (1991). Preparation and isolation of poly (3-hydroxyalkkanoates) obtained from Pseudomonas oleovorans grown with mixture of 5-phenylvaleric acid and n-alkanoic acids. Macromolecules. 24: 5256-5260.

Kunioka, M., Y. Kawaguchi and Y. Doi. (1989). Production of biodegradable copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate by Alcaligenes eutrophus. Appl Microbial Biotechnol 30: 569-573.

Law, J.H. and R.A. Slepecky. (1961). Assay of poly–hydrobutyric acid. J. Bacteriol. 82:33-36.

Lee, S.Y. and H.N. Chang. (1995a). "Effect of growth temperature and nutrients on the synthesis of poly (3-hydroxybutyric acid) by filamentation -suppressed recombinant E. coli", Annual Meeting of KIBB, KAIST.

Lee, S.Y. and H.N. Chang. (1995b). Production of poly(hydroxyalkanoic acid). Adv Biochem Eng Biotechnol. 52: 27-58.

Manchak, J., J.P. William and B. Rudy. (1995). Formation of Poly (Hydroxybutyrate-Co-Hydroxyvalerate) by Azotobacter Vinelandii UWD. Appl Environ Microbiol. 58(9): 2866-2873.

Mun, H.C., J.J. Song and S.C. Youn. (1995). Biosynthesis of copolyesters by Hydrogenophaga pseudoflava from various lactones. Can J Microbiol. 41(1): 60-67.

Omar, S., A. Rayes, A. Eqaab, I. Voß and A. Steinbüchel. (2001). Optimization of cell growth and poly(3-hydroxybutyrate) accumulation on date syrup by a Bacillus megaterium strain. 23(14): 1119-1123.

Page, W.J. and A. Cornish. (1993). Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of Poly-hydroxybutyrate. Appl Environ Microbiol. 59(12): 4236-2344.

Pfeffer, J.T. (1992). Recycling. Solid Waste Management Engineering 1992: 72-84.

Poirier, Y., C. Nawrath and C. Somerville. (1995). Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants. Biotechnol. 13: 142-150.

Rhee, Y.H., J.H. Jang and P.I. Rogers. (1993). Production of copolymer consisting of 3-Hydroxybutyrate and 3-Hydroxyvalerate by Fed- Batch Culture of Alcaligenes sp. SH-69. Biotechnol Letters. 15(4): 377-382.

Rusendi, D. and J.D. Sheppard. (1995). Hydrolysis of potato processing waste for the production of poly-hydroxybutyrate. Bioresource Technology 54(1995): 191-196.

Salehizadeh, H. and M.C.M. Loosdrecht. (2004). Production of polyhydroxyalkanoates by mixed culture: recent trends and biotechnological importance. Biotechnol Adv. 22: 261-279.

Sang, Y.L. (1996) Bacterial Polyhydroxyalkanoates. Biotechnol and Bioengn. 49 (1): 1-14.

Saruul, P., F. Srienc, A. David, D.A. Somers and Samac. (2002). Production of a Biodegradable Plastic Polymer, Poly-β-Hydroxybutyrate, in Transgenic Alfalfa. Crop Sc. 42 (3): 919-927.

Seon, W.K., P. Kim, H.S. Lee and K.H. Jung. (1996). High production of poly-hydroxybutyrate(PHB) from Methylobacterium organophilum under potassium limitation. Biotechnology Letters. 18(1): 25-30.

Silva, L.F., M.K. Taciro, M.E.M. Ramos, J.M. Carter, J.G.C. Pradella and J.G.C. Gomez. (2004). Poly-3-hydroxybutyrate (P3HB) production by bacteria from xylose, glucose and sugarcane bagasse hydrolysate. J Indust Microbiol and Biotechnol. 31(6): 1476-5535.

Steinbuchel, A. (1991). Polyhydroxyalkanoic acids. In Biomaterials, pp. 123-213. Edited by D. Byrom. Basingstoke, UK: Macmillan.

Tamer, I.M., Y. Chisti and M. Moo-Young. (1998). Disruption of Alcaligenes latus for recovery of poly(b-hydroxybutyric acid): Comparison of high-pressure homogenization, bead milling, and chemically induced lysis. Ind. Eng. Chem. Research. 37: 1807-1814.

Tina, L., B. Klaus, L. Heinrich and S. Alexander. (2001). Identification of a new class of biopolymer: bacterial synthesis of a sulfur-containing polymer with thioester linkages Microbiol. 147:11-19.

Van den Berg, D.J.C., G.W. Robijn, A.C. Janssen, M.L.F. Giuseppin, R. Vreeker, J.P. Kamerling, J.F.G. Vliegenthart, A.M. Ledeboer and C.T. Verrips. (1995). Production of a novel extracellular polysaccharide by Lactobacillus sake 0-1 and characterization of the polysaccharide. Appl Environ Microbiol. 61:2840-2844.

William, J.P. and K. Olga. (1989). Hyperproduction of Poly-β-Hydroxybutyrate during Exponential Growth of Azotobacter Vinelandii UWD. Appl Environ Microbiol. 55(6):334-1339.

Wong, A.L., H. Chua and P.H.E. Yu. (2000). Microbial production of polyhydroxyalkanoates by bacteria isolated from oil wastes. In Twenty-First Symposium on Biotechnology for Fuels and Chemicals (pp. 843-857). Humana Press, Totowa, NJ.




DOI: https://doi.org/10.33687/jpe.001.01.3577

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Dr. Muhammadi, Shabina Shafiq

Journal of Plant and Environment
ISSN: 2710-1665 (Online), 2710-1657 (Print)
© EScience Press. All Rights Reserved.