Counter Effect of Trichoderma harzianum Rifai. Against Cr (VI)

Sundus Akhtar, Ayesha Shafqat, Syeda Mariam Sherazi, Sonia Aslam, Habiba Khalil, Rubina Shakir, Muhammad Arslan Khan


Industrial and sewage water ejection in river and streams on daily basis and wide use of heavy metal is contaminating our surroundings. Among all the valences, Cr (VI) is regarded as a hazardous ion, which contaminates groundwater and can be transferred through the food chain In-vitro study was carried out in laboratory in which impact of Cr (VI) on Trichoderma harzianum was studied. For those two experiments i.e., solid medium assay and liquid broth assay were conducted. In solid medium assay, the radial growth, morphological alterations (appearance of colony, changes in the morphologies of spores and hyphae) in T. harzianum and inhibition zone under the stress of Cr (VI) at different doses i.e., 0, 1000, 2000, 3000, 4000 and 5000 ppm were studied. The results showed that radial growth was insignificantly reduced at highest dose (5000 ppm) i.e., 3.3% as compared to control. Moreover, no inhibition zone formed, and the mycelial pattern of tested fungi was crossing the disc zones. However, at 5000 ppm of Cr (VI), about 0.2 mm of concentric zone was observed. While no phialides were observed at higher doses of Cr (VI). Furthermore, in liquid broth experiment, mycelial growth, and biochemical attributes [Total protein content (TPC), Peroxidase (POX) and Catalase (CAT) activities] were studied after 7 days of incubation. The results revealed that the fresh and dry weight of T. harzianum was increased up to -200% comparison to control. The level of TPC, POX AND CAT increased by -10 % to -94% with the increasing concentration of Cr (VI) i.e., 1000 to 5000 ppm. Thus, the findings showed that T. harzianum could be used as bioremidier against Cr (VI). Further in- situ studies need to be taken to eradicate the presence of heavy metals in the environment by using fungus for bioremediation purpose in future.


Heavy metal; Cr (VI); Trichoderma harzianum; Growth; Antioxidant ezymes

Full Text:



Akhtar, S., and A. Shoaib. 2020. The counter defence system of antioxidants in Coelomycetous emerging human and plant pathogenic fungus Macrophomina phaseolina against copper toxicity. Environmental Science and Pollution Research, 27: 597-606.

Alyazeedi, A., A. F. Algendy, M. Sharabash and A. Karawia. 2019. Prevalence, determinants and associated risk of potentially inappropriate prescribing for older adults in Qatar: a national retrospective study. Clinical interventions in aging, 14, 1889.

Ambiental, E. Y. G. 2010. Trichoderma spp. and its potential in soil bioremediation. In United Nations Convention to Combat Desertification. European Commission.

Ayad, F., A. Matallah-Boutiba, O. Rouane–Hacene, M. Bouderbala and Z. Boutiba. 2018. Tolerance of Trichoderma sp. to heavy metals and its antifungal activity in Algerian marine environment. Journal of Marine Biology & Oceanography, 7(3), 2.

Beers, R. F., and I. W. Sizer. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of biological chemistry, 195(1), 133-140.

J. C. De Padua. 2021. Isolation and Characterization of Nickel-Tolerant Trichoderma Strains from Marine and Terrestrial Environments. Journal of Fungi, 7(8), 591.

Kapoor, A., T. Viraraghavan, D.R. Cullimore. 1999. Removal of heavy metals using fungus Aspergillus niger. Bioresorces Technology 70: 95-104.

Karcprzak, M., G. Malina. 2005. The tolerance and Zn2+, Ba2+ and Fe2+ accumulation by Trichoderma atroviride and Mortierella exigua isolated from contaminated soil. Canadian Journal of Soil Science 85: 283-290.

Khan, E., and M.A. Sajad. 2013. Phytoremediation of Heavy Metals—Concepts and Applications. Chemosphere, 91, 869-881.

Khan, M. U., R. N. Malik and S. Muhammad. 2013. Human health risk from heavy metal via food crops consumption with wastewater irrigation practices in Pakistan. Chemosphere, 93(10), 2230-2238.

López Errasquín. E., and C. Vázquez. 2003. Tolerance and uptake of heavy metals by Trichoderma atroviride isolated from sludge. Chemosphere 50: 137-143.

Lowry, O. H., Rosebrough. N. J, A. L. Farr and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193, 265-275.

Mishra, N., S. S. Khan and S. K. Sundari, 2016. Native isolate of Trichoderma: a biocontrol agent with unique stress tolerance properties. World Journal of Microbiology and Biotechnology, 32(8), 1-23.

Sarkar, S., k. A. Sathesh and R. Premkumar. 2013. Hexavalent chromium (Cr (VI)) removal by live mycelium of a Trichoderma harzianum strain. Molecular Soil Biology, 4(1).

Shoaib, J. Ahmed., A. Sundus and A. A. Zoia. 2019. Comparative resistance of maize cultivars to charcoal rot disease. Pakistan Journal of Science, 71: 202-207.

Singh, R., N. Gautam , A, Mishra and R. Gupta. 2011. Heavy metals and living systems: An overview. Indian journal of pharmacology, 43(3), 246.



  • There are currently no refbacks.

Copyright (c) 2023 Sundus Akhtar, Ayesha Shafqat, Syeda Mariam Sherazi, Sonia Aslam, Habiba Khalil, Arshad Rasool

Journal of Plant and Environment
ISSN: 2710-1665 (Online), 2710-1657 (Print)
© EScience Press. All Rights Reserved.