Odile Moullet, Arnold Schori


Marker-assisted selection (MAS) is a powerful tool too rarely exploited in practical breeding applications mainly because of its prohibitive costs. A new manual protocol has been developed for DNA extraction and polymerase chain reaction (PCR) analyses which could increase the impact of this technology on the creation of new varieties. In this procedure, only the amount of DNA serving as template is extracted directly into PCR tubes. The method is reproducible (100 %) and efficient (97.9 %). The overall cost is low in term of starting lab equipment (25000 €), chemicals and consumable materials (0.33 to 0.40 € per samples) and labor (1500 sample analyses per person and per week).


Marker-assisted selection, plant breeding, wheat, triticale.

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Journal of Plant Breeding and Genetics
ISSN: 2305-297X (Online), 2308-121X (Print)
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