Combining ability analysis and gene action for yield and yield related traits in rice (Oryza sativa L.) under saline conditions

Sieh S. Kargbo, Francis Showemimo, Pius Akintokun, Justina Porbeni


Salinity is a major abiotic constrain faced by farmers in most rice cultivating areas of the world and improving grain yield in rice is the most important breeding objective. Twenty seven hybrids were generated in a line x tester mating design and were evaluated with their parents in a Randomized Complete Block Design at the Fadama site of the Federal University of Agriculture, Abeokuta (FUNAAB) during the 2014/2015 and 2015/2016 cropping season. Analysis of variance revealed a highly significant difference (P<0.01) among testers and lines for all traits except panicle length and a number of effective tillers per plant, respectively. Variances of Specific Combining Ability (SCA) were higher in magnitude than the corresponding General Combining Ability (GCA). The lines FARO 60 (P7), OG300315 (P10), NERICA L53 (P4) including a tester ITA 212 (P1) were the best general combiners for yield per plant. POKKALI (P3) was the best general combiner for reduced vegetative growth and ITA 212 (P1) and ITA 222 (P2) were the best general combiners for plant height. The best specific combiner for yield per plant was P1 x P4. P3 x P11 was the best specific combiner for reduced duration characters and P2 x P7 was the best specific combiner for plant height.  Estimates of narrow sense heritability (0.00 – 0.03) for all the traits under study were low which indicated preponderance of non-additive gene action governing these traits. Therefore, inter-mating among selected segregants followed by recombination breeding in an advanced generation might be advocated for improvement of the studied traits under salinity.


Rice (Oryza sativa L.); Line x tester; Specific Combining Ability; General Combining Ability; gene action; salinity tolerance

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Ahmadikhah A, (2008). Estimation of heritability and heterosis of some agronomic traits and combining ability of rice lines using line x tester method. Electronic Journal of Crop Production 1(2): 15-33.

Aslam R, Munawar M, Salam A (2014). Genetic architecture of yield components accessed through Line x Tester analysis in wheat (Triticum aestivum L.) Universal Journal of Plant Science 2: 93-96.

Borghi B, Perenzin M, (1994). Diallel analysis to predict heterosis and combining ability for grain yield, yield components and bread-making quality in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 89 (7-8): 975 - 981.

Devi A, Kumari P, Dwivedi R, Dwivedi S, Mishra KK, Verma OP, Singh PK, Dwivedi DK, (2017). Combining ability analysis for yield and its quality traits in rice (Oryza sativa L.) over environment. Journal of Pharmacognosy and Phytochemistry 6(4): 35 - 42

Falconer DS, Mackay TFC (1996). Introduction to Quantitative Genetics, 4th edition, Longman, Harlow, Essex, UK.

Fahmi AI, Eissa Ragaa A, Nagaty HH, El-Malky M, Sherif AI (2017). Genetic components and correlation coefficient for earliness and grain yield in rice. Vegetos 30:4

Fellahi ZEA, Hannachi A, Bouzerzour H, Boutekrabt A (2013). Line × tester mating design analysis for grain yield and yield related traits in bread wheat (Triticum aestivum L.). International Journal of Agronomy 1–9

Gholizadeh AG, Nematzadeh G, Bagheri N, Oladi M, Bagheri A (2014). Heritability and heterosis of agronomic traits in rice lines. International Journal of Farming and Allied Sciences 3(1):66 - 70

Gnanasekaran M, Vivekanandan P, Muthuramu S, (2006). Combining ability and heterosis for yield and grain quality in two line rice (Oryza sativa L.) hybrids. Indian Journal of Human Genetics 66: 6 - 9.

Gopikannan M, Ganesh SK (2013). Investigation on combining ability and heterosis for sodicity tolerance in rice (Oryza sativa L.). African Journal of Agricultural Research 8(32): 4326 – 4333

Kempthorne O. 1957. An introduction to genetic statistics. John Wiley & Sons.

Koze A, (2017). Gene action and combining ability in line x tester population of safflower (Carthamus tinctorious L.). Field crops 22 (2): 197 – 203

Kumar M, Kumar K, Verma GP, Verma OP (2010). Combining ability analysis for yield and components traits under saline alkaline soil in rice. Oryza, 47 (3): 193 - 200

Manickavelu A, Gnanamalar RP, Nadarajan N, Ganesh SK (2006). Genetic variability studies on different genetic populations of rice under drought condition. Journal of Plant Sciences 1(4): 332-339.

Patial M, Pal D, Kumar J (2016). Combining ability and gene action studies for grain yield and its components traits in Barley (Hordeum vulgare L.). SABRAO Journal of Breeding and Genetics 48(1): 90 – 96.

Peng JY, Virmani SS (1990). Combining ability for yield and four yield related traits in relation to breeding rice. Oryza 27: 1-10

Pradhan SK, Boss LK, Meher J (2006). Studies on gene action and combining ability analysis in Basmati rice. Journal of Central European Agriculture 7(2): 267-272.

Premlatha M, Kalamani A, Nirmalakumari A (2011). Heterosis and combining ability for grain yield and quality in maize (Zea mays L.). Advances in Environmental Biology 5(6): 1264–1266.

Rahaman A, (2016). Study of nature and magnitude of gene action in hybrid rice (Oryza sativa L.) through experiment of line x tester mating design. International Journal of Applied Research 2(2):405-410.

Sarma MK, Sharma AK, Agrawal RK, Richharia AK (2007). Combining ability and gene action for yield and quality traits in Ahu rices of Assam. Indian Journal of Genetics and Plant Breeding 67 (3): 278-280.

Shanthi P, Jebaraj S, Geetha S (2011). Study on gene action for sodic tolerance traits in rice (Oryza sativa L.). Electronic Journal of Plant Breeding 2(1): 24-30.

Simmonds NW (1989). How frequent are superior genotypes in plant breeding populations. Biological Review 64: 341-365.

Singh R, Singh H (1985). Combining ability and heterosis for seed yield, its component characteristics in indian mustard sown early and late. Indian Journal Agricultural Sciences 55: 309 - 315.

Singh RJ, Maurya A (1999). Evaluation of CMS lines for various floral traits influence outcrossing in rice. International Rice Research Notes 28: 24-26.

Thirumalai R, Palaniraja K, Vennila S (2018). Yield response of rice genotypes for gene action under coastal saline condition. International Journal of Current Microbiology and Applied Sciences 7(4): 3353 – 3360

Vanave PB, Vaidya GB, Jadhav BD (2018). Combining ability of rice genotypes under coastal saline conditions. Electronic Journal of Plant Breeding 9(1): 116-123

Zhang X, Liangiie LV, Chai LV, Baojian G, Rugen X (2015). Combining ability of different agronomic traits and yield components in hybrid barley. Plos One 10: 6



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